p38 Mitogen-activated protein kinase regulation of endothelial cell migration depends on urokinase plasminogen activator expression.

The Journal of Biological Chemistry
Jianqiang YuShuang Huang

Abstract

The migration of endothelial cells in response to various stimulating factors plays an essential role in angiogenesis. The p38 MAPK pathway has been implicated to play an important role in endothelial cell migration because inhibiting p38 MAPK activity down-regulates vascular endothelial growth factor (VEGF)-stimulated migration. Currently, the signaling components in the p38 MAPK activation pathway and especially the mechanisms responsible for p38 MAPK-regulated endothelial cell migration are not well understood. In the present study, we found that p38 MAPK activity is required for endothelial cell migration stimulated by both VEGF and nongrowth factor stimulants, sphingosine 1-phosphate and soluble vascular cell adhesion molecule. By using dominant negative forms of signaling components in the p38 MAPK pathway, we identified that a regulatory pathway consisting of MKK3-p38alpha/gamma-MAPK-activated protein kinase 2 participated in VEGF-stimulated migration. In further studies, we showed that a minimum of a 10-h treatment with SB203580 (specific p38 MAPK inhibitor) was needed to block VEGF-stimulated migration, suggesting an indirect role of p38 MAPK in this cellular event. Most interestingly, the occurrence of SB203580-induce...Continue Reading

References

Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Feb 17, 2000·Cellular Signalling·K Ono, J Han
Jun 6, 2000·Trends in Biochemical Sciences·A R Nebreda, A Porras
Jul 6, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Martín-Blanco
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S MudgettM M Shen
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q Wang, C M Doerschuk
Jul 10, 2001·The Journal of Clinical Endocrinology and Metabolism·P KoolwijkV W van Hinsbergh
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M O HanniganC K Huang
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eunkyung Lee, Pietro De Camilli
Mar 12, 2002·The Journal of Investigative Dermatology·W LiD Woodley
Jun 8, 2002·Molecular and Cellular Biology·Alexey KotlyarovMatthias Gaestel
Jun 11, 2002·British Journal of Pharmacology·László DénesPéter Csermely
Jun 19, 2002·Biochimica Et Biophysica Acta·Tracee Scalise Panetti
Aug 31, 2002·The Journal of Biological Chemistry·Usamah S KayyaliPaul M Hassoun
Sep 20, 2002·The Journal of Biological Chemistry·Amit VermaLeonidas C Platanias
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shintaro NakaoMayumi Ono
Dec 4, 2003·Biochemical Society Transactions·I Zachary
Jan 30, 2004·Cancer Cell·Mikhail V Blagosklonny

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Citations

Mar 3, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jincai LuoYao Lu
Jun 7, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Fei WangXiaoxin Li
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yung-Ming ChangChih-Yang Huang
Jul 26, 2011·Fiziologiia cheloveka·S A Kryzhnovskiĭ, M B Vititnova
Dec 25, 2012·Genes & Cancer·Yusuke KobayashiGuan Chen
Oct 8, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Yung-Ming ChangChih-Yang Huang
Apr 3, 2009·Biochemical and Biophysical Research Communications·Eugenio JiménezMercedes Montiel
Apr 8, 2017·Journal of Molecular Endocrinology·Natalia CalvoClaudia Gentili
Sep 14, 2013·Angewandte Chemie·Tiago RodriguesGisbert Schneider
Mar 31, 2007·Circulation Research·Laurent LamaliceJacques Huot
Jan 23, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Basak IcliMark W Feinberg
Jan 9, 2020·American Journal of Physiology. Cell Physiology·Basak IcliMark W Feinberg
Oct 5, 2018·Journal of Experimental & Clinical Cancer Research : CR·Changliang ChenShuang Huang

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