p42/p44 MAP kinase module plays a key role in the transcriptional regulation of the vascular endothelial growth factor gene in fibroblasts.

The Journal of Biological Chemistry
J MilaniniGilles Pages

Abstract

Vascular Endothelial Growth Factor (VEGF) is a potent mitogen for vascular endothelial cells that has been implicated in tumor neovascularization. We show that, in hamster fibroblasts (CCL39 cells), VEGF mRNAs are expressed at low levels in serum-deprived or exponentially growing cells, whereas it is rapidly induced after stimulation of quiescent cells with serum. CCL39 derivatives, transformed with Polyoma virus or with active members of the p42/p44 mitogen-activated protein (MAP) kinase pathway, Gly/Val point mutant of Ras at position 12 (Ras-Val12), MKK1 in which Ser218 and Ser222 were mutated to Asp (MKK1-SS/DD)), express very high levels of VEGF mRNA. To analyze the contribution of the p42/p44MAP kinase in this induction, we used the CCL39-derived cell line (Raf-1:ER) expressing an estradiol-activable Raf-1. We show a time and an estradiol dose-dependent up-regulation of VEGF mRNA clearly detectable after 2 h of stimulation. The induction of VEGF mRNA in response to conditioned activation of Raf-1 is reverted by an inhibitor of MKK1, PD 098059, highlighting a specific role for the p42/p44 MAP kinase pathway in VEGF expression. Interestingly, hypoxia has an additive effect on VEGF induction in CCL39 cells stimulated by seru...Continue Reading

References

Jan 1, 1992·Advances in Cancer Research·C Basilico, D Moscatelli
Feb 14, 1991·Biochemical and Biophysical Research Communications·M ToiR Bicknell
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Jun 15, 1989·Biochemical and Biophysical Research Communications·N Ferrara, W J Henzel
Feb 1, 1971·The Journal of Experimental Medicine·J FolkmanG Williams
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J PouysségurS Paris
Feb 16, 1995·Nature·T Pawson
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·A Gashler, V P Sukhatme
Jun 2, 1995·The Journal of Biological Chemistry·A P LevyM A Goldberg
Sep 1, 1994·Current Biology : CB·T KodakiP J Parker
Mar 8, 1995·Biochemical and Biophysical Research Communications·G FinkenzellerD Marmé
Aug 5, 1994·Science·Z Galcheva-GargovaR J Davis
Aug 18, 1994·Nature·P Rodriguez-VicianaJ Downward
Jan 12, 1996·The Journal of Biological Chemistry·T CohenB Z Levi
Feb 2, 1996·The Journal of Biological Chemistry·A P LevyM A Goldberg
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·B OlofssonU Eriksson
Jun 28, 1996·The Journal of Biological Chemistry·P LenormandJ Pouysségur
Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Apr 17, 1997·Nature·W Risau
Dec 1, 1996·Trends in Cell Biology·G Breier, W Risau

❮ Previous
Next ❯

Citations

Jan 2, 2003·Annals of Neurology·Candelaria Gomez-ManzanoW K Alfred Yung
Apr 17, 2001·International Journal of Cancer. Journal International Du Cancer·R MentleinJ Held-Feindt
Apr 23, 2002·Journal of Cellular Biochemistry·Qiang ZhangElaine Unemori
Mar 5, 2004·International Journal of Colorectal Disease·Markus GubaChristiane Bruns
Jul 16, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Alexa Klettner, Johann Roider
Sep 10, 2008·Apoptosis : an International Journal on Programmed Cell Death·Zhonghan YangGuoquan Gao
Nov 12, 2005·Molecular and Cellular Biochemistry·Weiming OuyangChuanshu Huang
Mar 9, 2013·Cell Biochemistry and Biophysics·Ming FangWenlong Huang
May 20, 2009·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·F MorgilloF Ciardiello
Feb 27, 2004·Biochimica Et Biophysica Acta·Onno KranenburgEmile E Voest
Mar 22, 2005·Gene·Shijian Chu, Thomas J Ferro
Mar 17, 2004·Seminars in Cancer Biology·Janusz Rak, Joanne L Yu
Sep 29, 2000·Biochemical Pharmacology·E BerraJ Pouysségur
Nov 28, 2002·Biochimie·Sumiko WatanabeKen-ichi Arai
Mar 15, 2000·Molecular and Cellular Endocrinology·B D RichardsonS Handwerger
Jan 1, 2003·Cancer Detection and Prevention·Regina Maki SasaharaMari Cleide Sogayar
Nov 22, 2001·The Journal of Steroid Biochemistry and Molecular Biology·L BermontG L Adessi
Dec 28, 1999·Trends in Cell Biology·F McCormick
Aug 9, 2002·Oral Oncology·Pornchai O-charoenratSuzanne A Eccles
Oct 14, 2011·Nature Reviews. Cancer·Yuliya Pylayeva-GuptaDafna Bar-Sagi
Feb 27, 2007·Oncogene·P E DepeilleN S Duesbery
Nov 28, 2002·Journal of Gastroenterology and Hepatology·Shih-Pei HuangJaw-Town Lin
Nov 14, 2002·Molecular Biology of the Cell·Lily YenMoulay A Alaoui-Jamali
Feb 29, 2008·Nucleic Acids Research·Amandine BastideChristian Touriol
Jun 8, 2002·Molecular and Cellular Biology·Akihiro TakahashiKenneth Walsh
Jun 1, 2004·Molecular and Cellular Biology·Naohiro WakisakaJoseph S Pagano
Nov 2, 2005·Molecular and Cellular Biology·Jun WangMona Nemer
Oct 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Munenori TakaokaHiroshi Nakagawa
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Thomas Hellwig-BürgelWolfgang Jelkmann
Oct 9, 2002·The Journal of Clinical Investigation·Gaël NicolasSophie Vaulont
Mar 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Richard T KloosManisha H Shah

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.