PMID: 9630546Jun 19, 1998Paper

P450 isoforms in a murine macrophage cell line, RAW264.7, and changes in the levels of P450 isoforms by treatment of cells with lipopolysaccharide and interferon-gamma

Biochimica Et Biophysica Acta
M NakamuraY Funae

Abstract

The presence of P450 in a murine macrophage cell line, RAW264.7, was investigated to clarify the biological role and regulation of P450. Microsomes of RAW264.7 cells were isolated and subjected to immunoblotting with anti-rat CYP2A1, 2B1, and 4A2 antibodies. The microsomes gave staining bands with all these antibodies, suggesting the presence of mouse Cyp2a, 2b, and 4a isoforms in RAW264.7. RAW264. 7 cells were treated with typical inducers of P450 (phenobarbital, clofibrate, beta-naphthoflavone and 3-methylcholanthrene). None of these chemicals induced these P450s. Stimulation of RAW264.7 cells with lipopolysaccharide (LPS) and interferon-gamma (INF-gamma) which increase inducible nitric oxide synthase (iNOS) and cytokines in cells decreased Cyp4a protein but not Cyp2a and 2b proteins. To identify P450 isoforms in RAW264.7, we used polymerase chain reaction (PCR) primers for mouse Cyp2a4, 2a12, 2b9/10, 4a10, and 4a12. Total RNA was isolated from these cells and converted to cDNA by reverse transcriptase. PCR was done with these primers and the amplified nucleotides were analyzed by a DNA sequencer. Only Cyp2b9/10 and 4a12 primers gave clear bands, although all primers gave clear bands from liver total RNA. Nucleotide sequences...Continue Reading

References

Jul 28, 1992·Biochemistry·K A White, M A Marletta
Sep 16, 1992·Biochemical and Biophysical Research Communications·J L BoucherD Mansuy
Apr 1, 1990·Archives of Biochemistry and Biophysics·S ImaokaY Funae
Oct 26, 1995·Biochemical Pharmacology·D R GermolecM I Luster
Apr 7, 1995·Atherosclerosis·M I DushkinN N Volsky
Oct 14, 1996·Biochemical and Biophysical Research Communications·Y SohB J Song

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Citations

Oct 23, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Qing-Yu ZhangLaurence S Kaminsky
Jun 28, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yuki TochigiHiroshi Yokota

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