p48 Activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity.

Molecular and Cellular Biology
B J HwangG Chu

Abstract

A subset of xeroderma pigmentosum (XP) group E cells lack a factor that binds to DNA damaged by UV radiation. This factor can be purified to homogeneity as p125, a 125-kDa polypeptide. However, when cDNA encoding p125 is translated in vitro, only a small fraction binds to UV-damaged DNA, suggesting that a second factor is required for the activation of p125. We discovered that most hamster cell lines expressed inactive p125, which was activated in somatic cell hybrids containing human chromosome region 11p11.2-11cen. This region excluded p125 but included p48, which encodes a 48-kDa polypeptide known to copurify with p125 under some conditions. Expression of human p48 activated p125 binding in hamster cells and increased p125 binding in human cells. No such effects were observed from expression of p48 containing single amino acid substitutions from XP group E cells that lacked binding activity, demonstrating that the p48 gene is defective in those cells. Activation of p125 occurred by a "hit-and-run" mechanism, since the presence of p48 was not required for subsequent binding. Nevertheless, p48 was capable of forming a complex with p125 either bound to UV-damaged DNA or in free solution. It is notable that hamster cells fail to...Continue Reading

References

Mar 29, 1991·Biochemical and Biophysical Research Communications·H Kataoka, Y Fujiwara
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G Chu, E Chang
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R RuggieriK Matsumoto
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Oct 27, 1995·The Journal of Biological Chemistry·Y W Qian, E Y Lee
Jul 1, 1994·Molecular and Cellular Biology·W K Rathmell, G Chu
Dec 23, 1994·Science·P C Hanawalt
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·W K Rathmell, G Chu
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KeeneyS Linn
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·A KazantsevA Sancar
May 17, 1996·The Journal of Biological Chemistry·J L BurnsL Prakash
Oct 4, 1996·The Journal of Biological Chemistry·A F NicholsS Linn

❮ Previous
Next ❯

Citations

Sep 24, 2016·Quarterly Reviews of Biophysics·Sandra C KochThomas Carell
Jun 21, 2011·DNA Repair·Hanspeter Naegeli, Kaoru Sugasawa
Dec 22, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anne LagerqvistDag Jenssen
Aug 30, 2005·Journal of Photochemistry and Photobiology. B, Biology·Photini PitsikasAndrew J Rainbow
Dec 27, 2008·Cell·Andrea ScrimaNicolas H Thomä
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Jan 31, 2004·Biochemical and Biophysical Research Communications·Taeko InokiHitoshi Endo
Apr 24, 2008·PloS One·Zilal KattanPhilippe Becuwe
May 28, 2010·BMC Cancer·Marcus Q BernardiniAndrew Berchuck
Aug 25, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ning ZouQi-En Wang
Aug 28, 2012·Radiation Protection Dosimetry·M Chiba
May 10, 2012·Nucleic Acids Research·Manpreet KaurSandeep Saxena
Dec 6, 2008·Nucleic Acids Research·Mitsuo WakasugiTsukasa Matsunaga
Oct 12, 2011·Acta Biochimica Et Biophysica Sinica·Jia LiWei Xiao
Jan 15, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nur DualeGunnar Brunborg
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·B J HwangG Chu
Mar 11, 2005·Biology of Reproduction·Guogang XuChristi A Walter
Feb 26, 1999·Environmental and Molecular Mutagenesis·Z YuB W Glickman
Dec 18, 2001·Environmental and Molecular Mutagenesis·P C Hanawalt
Oct 31, 2012·International Journal of Molecular Sciences·Nilotpal RoyPradip Raychaudhuri
Dec 3, 2004·Molecular Biology of the Cell·Jeroen EssersRoland Kanaar
Feb 24, 2006·Environmental and Molecular Mutagenesis·Huxian WangJohn B Hays
Dec 18, 2013·Journal of Virology·Xiaofei ETimothy F Kowalik
Feb 18, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Michal LiebermanIlan Levin
Aug 2, 2005·The Journal of Biological Chemistry·Alvin T C LeeCaroline G L Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.