P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts

Archives of Virology
Xiao-Ya LiuHeng-Mu Zhang

Abstract

The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.

References

May 25, 1999·Protein Science : a Publication of the Protein Society·O EmanuelssonG von Heijne
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Aug 10, 2005·Journal of Experimental Botany·Jocelyn Bédard, Paul Jarvis
Sep 4, 2007·Advances in Virus Research·Christopher NethertonThomas Wileman
Jun 19, 2008·The Journal of General Virology·Chaozheng ZhangBingsheng Qiu
Sep 30, 2008·Archives of Virology·Heng-Mu ZhangM J Adams

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