PMID: 9442379Jan 27, 1998Paper

p50 (NF-kappa B1) is upregulated in LPS tolerant P388D1 murine macrophages

Immunobiology
H W Ziegler-HeitbrockM Frankenberger

Abstract

Cells of the murine macrophage cell line P388D1 express cell surface CD14 and respond to LPS (lipopolysaccharide) stimulation with the production of TNF (tumor necrosis factor). When the cells are stimulated with LPS a second time then little TNF is produced, i.e. the cells are tolerant. Flow cytometry analysis demonstrates that this tolerance is not due to a downregulation of the CD14 cell surface receptor. Analysis of proteins binding to the -516 NF-kappa B motif of the murine TNF promoter reveals that constitutive p50p50 and LPS stimulation lead to mobilization of a heterodimer consisting of p65/c-rel. In tolerant cells less of the p65/c-rel heterodimer is mobilized but there is a strong upregulation of p50p50. These data show that tolerance to LPS in murine macrophages may involve a predominance of p50 homodimers.

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J G HaasH W Ziegler-Heitbrock
Mar 1, 1993·Immunology Today·H W Ziegler-Heitbrock, R J Ulevitch
Dec 1, 1994·The Journal of Experimental Medicine·K E LaRue, C E McCall
Nov 15, 1994·The Biochemical Journal·M FrankenbergerH W Ziegler-Heitbrock
Jan 1, 1993·Immunology Today·B ArnoldG J Hämmerling

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Citations

Oct 27, 2009·Inflammatory Bowel Diseases·Andre BleichEdward H Leiter
Dec 14, 1999·Biological Chemistry·A WedelH W Ziegler-Heitbrock
Mar 17, 2016·Journal of Immunology Research·Alex Steimle, Julia-Stefanie Frick
May 18, 1999·The Journal of Surgical Research·A A KramerC Mendez
Nov 16, 2002·Immunity·Ichiko KinjyoAkihiko Yoshimura
Mar 9, 2004·Biochemical and Biophysical Research Communications·Ruth C BorghaeiJoanna Woloshin
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M WysockaC L Karp
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A E MedvedevS N Vogel
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mihaela GadjevaBruce H Horwitz
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B A ButcherE Y Denkers
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam J GehringW Henry Boom
Dec 18, 2003·Clinical and Experimental Immunology·F DriesslerA J Schottelius

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