PMID: 18719376Aug 23, 2008Paper

p53 Binding to the p21 promoter is dependent on the nature of DNA damage

Cell Cycle
Richard HillPatrick W K Lee

Abstract

p53 Is a tumor suppressor that integrates signals from different stress induced signalling pathways, regulates cell cycle arrest, senescence, apoptosis and DNA repair. How p53 dictates cell fate is unclear. As a major transcriptional target of p53 in response to cellular stress, p21 is a key component in cell cycle control and apoptosis, directing an anti-apoptotic response following DNA damage. It is therefore likely that p53-dependent regulation of p21 contributes, at least in part, how p53 influences cellular outcome upon DNA damage. Here we compare the p53-dependent transcriptional regulation of p21 in response to DNA damage by ultraviolet (UV) radiation and ionizing radiation (IR). We demonstrate that despite comparable levels of p53 accumulation by both types of DNA damage, IR causes significant, early accumulation of p21 not seen in UV-damaged cells, with a substantially different cell cycle profile. Whereas UV and IR both target p21 protein for degradation immediately after DNA damage, differential post-damage p21 transcription is accountable for the disparity in p21 protein levels. Chromatin immunoprecipitation studies reveal that p53 displays a clear bias against the p21 promoter in UV-damaged cells compared to IR-dam...Continue Reading

Citations

Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jessica A BertoutM Celeste Simon
Feb 26, 2013·International Journal of Molecular Sciences·Patrícia A Madureira, David M Waisman
Aug 6, 2013·International Journal of Molecular Sciences·Ronald-Allan M PanganibanRegina M Day
Dec 15, 2010·International Journal of Otolaryngology·Wong-Kein LowMichelle G K Tan
Jun 16, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Priyatham Gorjala, Ronald K Gary
May 23, 2012·International Journal of Hygiene and Environmental Health·Vinita ChauhanRuth C Wilkins
Nov 8, 2014·Neoplasia : an International Journal for Oncology Research·Melissa N ScheiberDennis K Watson
Apr 10, 2010·Archives of Oral Biology·Monika Damek-PoprawaSunday O Akintoye
Sep 22, 2012·The Prostate·Chengfei LiuAllen C Gao
Oct 7, 2008·Mechanisms of Development·Nadia DanilovaShuo Lin
Oct 19, 2013·Biochemical and Biophysical Research Communications·Jan CoufalVáclav Brázda
Jun 5, 2014·American Journal of Physiology. Endocrinology and Metabolism·Daniel K FoxChristopher M Adams
Aug 9, 2018·Molecular Biology Reports·Angelika DługoszAnna Janecka
May 26, 2018·Cell Death & Disease·Eun-Seok ChoiSung-Ho Goh
Dec 10, 2009·Current Opinion in Oncology

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