p53 expression in the gastric mucosa before and after eradication of Helicobacter pylori

Helicobacter
K SatohK Sugano

Abstract

Accumulation of p53 has been recognized in the gastric mucosa infected with Helicobacter pylori. We investigated the prevalence of p53-positive cells in the gastric mucosa before and one month after eradication of H. pylori and the relationship between p53 positivity and inflammation and cell proliferation. The subjects included 24 H. pylori-positive patients. They achieved eradication one month after anti-H. pylori therapy. Biopsies were taken from the greater curvatures of the antrum and middle body. H. pylori status was assessed using culture and tissue section (Giemsa stain). Serial sections were used for examination of gastritis (hematoxylin and eosin stain) and for immunostaining of p53, Ki-67 and myeloperoxidase (MPO). p53 index and Ki-67 labeling index (LI) were calculated by counting p53-positive and Ki-67-positive cells in the entire gastric pits longitudinally sectioned and expressing them as a percentage of the total cells in a gastric pit. In the neck regions with and without p53-positive cells, polymorphonuclear leukocytes (PMNs) were counted in the corresponding area (/50 x 50 microm2) of the sections stained both with p53 and MPO. p53-positive cells decreased significantly after eradication of H. pylori. Before ...Continue Reading

References

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Citations

May 16, 2003·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·K SatohK Sugano
Jun 11, 2005·Journal of Gastroenterology and Hepatology·Masaaki KodamaMasaru Nasu
Dec 22, 2011·The Tohoku Journal of Experimental Medicine·Katsunori IijimaTooru Shimosegawa
Feb 4, 2010·Biochimica Et Biophysica Acta·Ana Manuel Dantas MachadoLene Juel Rasmussen
Mar 7, 2008·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Jiro WatariKiron M Das
Mar 6, 2003·Human Mutation·C M Fenoglio-PreiserA Noffsinger
Dec 23, 2004·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Masaaki KodamaToshio Fujioka
Jul 2, 2002·American Journal of Clinical Pathology·Cheryl M CoffinSharon Geaghan
Jan 27, 2006·World Journal of Gastroenterology : WJG·Masaaki KodamaToshio Fujioka
Apr 2, 2002·World Journal of Gastroenterology : WJG·Xin-Liang LuQin Du
Nov 29, 2016·Clinical Medicine Insights. Gastroenterology·Konstantinos TriantafyllouSpiros D Ladas

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