p53 in polyoma virus transformed REF52 cells

Oncogene
O MorMike Fried

Abstract

While cellular transformation by small DNA tumour viruses usually involves targeting the product of the p53 tumour suppressor gene by a virally encoded protein, none of the three polyoma virus (Py) specified T antigens have been observed to interact with p53. We show that primary mouse embryo fibroblasts and REF52 cells, which resemble primary cells in requiring co-operating oncogenes for transformation, cannot be transformed by the Py oncogene, middle T-antigen (PyMT), alone. These cells can be transformed by the complete Py early region, which encodes the Py large, middle and small T-antigens. We find that PyMT can transform rodent cells lacking a functional p53 protein (p53 null mouse embryo fibroblasts and DN-REF52 cells which contain a dominant negative p53). In Py transformed REF52 cells (Py-REF52) there is no significant accumulation of p53 protein, as opposed to SV40 transformed REF52 cells (SV-REF52) in which the amount of steady state p53 protein is elevated. However accumulation of p53 is observed following exposure of Py-REF52 cells to u.v. Treatment of Py-REF52 cells with X-rays results in a rapid increase in the levels of the p53-induced proteins p21/WAF1 and MDM2. In untransformed REF52 cells, X-irradiation cause...Continue Reading

Citations

Aug 21, 2002·Journal of Virology·Dilip DeyThomas L Benjamin
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·K A Gottlieb, L P Villarreal
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pablo Rodriguez-VicianaMike Fried
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Madeleine G MouleMike Fried
Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M L OliveiraM C Sogayar
Aug 30, 2001·Oncogene·M Lomax, M Fried
Dec 2, 1999·The Journal of General Virology·A Marti, K Ballmer-Hofer

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