p53-Independent induction of Fas and apoptosis in leukemic cells by an adenosine derivative, Cl-IB-MECA

Biochemical Pharmacology
S G KimK A Jacobson

Abstract

A(3) adenosine receptor (A(3)AR) agonists have been reported to influence cell death and survival. The effects of an A(3)AR agonist, 1-[2-chloro-6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-1-deoxy-N-methyl-beta-D-ribofuranonamide (Cl-IB-MECA), on apoptosis in two human leukemia cell lines, HL-60 and MOLT-4, were investigated. Cl-IB-MECA (> or =30 microM) increased the apoptotic fractions, as determined using fluorescence-activated cell sorting (FACS) analysis, and activated caspase 3 and poly-ADP-ribose-polymerase. Known messengers coupled to A(3)AR (phospholipase C and intracellular calcium) did not seem to play a role in the induction of apoptosis. Neither dantrolene nor BAPTA-AM affected the Cl-IB-MECA-induced apoptosis. Cl-IB-MECA failed to activate phospholipase C in HL-60 cells, while UTP activated it through endogenous P2Y(2) receptors. Induction of apoptosis during a 48hr exposure to Cl-IB-MECA was not prevented by the A(3)AR antagonists [5-propyl-2-ethyl-4-propyl-3-(ethylsulfanylcarbonyl)-6-phenylpyridine-5-carboxylate] (MRS 1220) or N-[9-chloro-2-(2-furanyl)[1,2,4]triazolo[1,5-c]quinazolin-5-yl]benzeneacetamide (MRS 1523). Furthermore, higher concentrations of MRS 1220, which would also antagonize A(1) and A(2A) re...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N R RodriguesD P Lane
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Wolf, V Rotter
Feb 1, 1995·Biology of Reproduction·S M QuirkK P Henrikson
Jun 1, 1995·Molecular and Cellular Biology·L B Owen-SchaubE Kruzel
Jul 1, 1995·British Journal of Pharmacology·J P HannonJ R Fozard
Feb 24, 1995·European Journal of Pharmacology·D K Von LubitzK A Jacobson
Feb 27, 1996·Biochemical and Biophysical Research Communications·Y KohnoK A Jacobson
Mar 18, 1996·European Journal of Pharmacology·J R FozardH J Schuurman
Mar 17, 1997·Biochemical and Biophysical Research Communications·Y YaoK A Jacobson
Jul 4, 1998·Trends in Pharmacological Sciences·K A Jacobson
Sep 23, 1998·Experimental Cell Research·V ShneyvaysA Shainberg
Mar 17, 1999·European Journal of Pharmacology·D K Von LubitzK A Jacobson
Mar 19, 1999·Biochemical and Biophysical Research Communications·Y R Chen, T H Tan
Jun 25, 1999·Cell Death and Differentiation·A N RakkarP Seth
Mar 24, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·R BrambillaM P Abbracchio

❮ Previous
Next ❯

Citations

Jun 15, 2007·Cancer Chemotherapy and Pharmacology·Hellinida ThomadakiAndreas Scorilas
Apr 12, 2008·Purinergic Signalling·Stefania MerighiPier Andrea Borea
May 6, 2011·Purinergic Signalling·Dennis Verzijl, Ad P Ijzerman
Apr 6, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Azmi YerlikayaEngin Ulukaya
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Mojtaba Panjehpour, Fatemeh Karami-Tehrani
Oct 11, 2003·Pharmacology & Therapeutics·Stefania MerighiPier Andrea Borea
Mar 7, 2006·Nature Reviews. Drug Discovery·Kenneth A Jacobson, Zhan-Guo Gao
Jul 5, 2008·Journal of Medicinal Food·Moon-Moo KimSe-Kwon Kim
Sep 28, 2010·American Journal of Respiratory and Critical Care Medicine·Katia VaraniPier Andrea Borea
Jul 20, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Petr DolezelPetr Mlejnek
Nov 14, 2006·Experimental Cell Research·Marina HermesDoris Kloor
Apr 6, 2007·British Journal of Haematology·Natarajan V BhanuJeffery L Miller
Sep 19, 2007·Expert Opinion on Emerging Drugs·Zhan-Guo Gao, Kenneth A Jacobson
Jul 17, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Antonella MontinaroSilvana Morello
Oct 5, 2010·Biochimica Et Biophysica Acta·Stefania GessiPier Andrea Borea
Feb 25, 2017·Journal of Cellular Physiology·Mohammad H KazemiFarhad Jadidi-Niaragh
Feb 13, 2003·The Journal of Pathology·Mahmoud R HusseinGary S Wood
Nov 15, 2013·Nature Reviews. Cancer·Luca AntonioliGyörgy Haskó
Sep 25, 2019·Cancers·Marcella CamiciMaria Grazia Tozzi
Jul 25, 2019·Current Pharmaceutical Design·Bapi GorainSubrat Kumar Bhattamisra
Apr 3, 2003·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·M R HusseinG S Wood
Aug 12, 2005·Molecular Cancer Therapeutics·Pilar García-MoralesMiguel Saceda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis