p53 is involved in clearance of ionizing radiation-induced RAD51 foci in a human colon cancer cell line

Biochemical and Biophysical Research Communications
Lukas M OrreJanne Lehtiö

Abstract

We have investigated p53-related differences in cellular response to DNA damaging agents, focusing on p53s effects on RAD51 protein level and sub-cellular localization post exposure to ionizing radiation. In a human colon cancer cell line, HCT116 and its isogenic p53-/- subcell line we show here p53-independent RAD51 foci formation but interestingly the resolution of RAD51 foci showed clear p53 dependence. In p53 wt cells, but not in p53-/- cells, RAD51 protein level decreased 48 h post irradiation and fluorescence immunostaining showed resolution of RAD51 foci and relocalization of RAD51 to nucleoli at time points corresponding to the decrease in RAD51 protein level. Both cell lines rejoined DNA double strand breaks efficiently with similar kinetics and p53 status did not influence sensitivity to DNA damaging agents. We suggest that p53 has a role in RAD51 clearance post DSB repair and that nucleoli might be sites of RAD51 protein degradation.

References

Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·O V KovalenkoE I Golub
Jan 24, 1997·Cell·R ScullyD M Livingston
Apr 8, 2000·Trends in Cell Biology·M O OlsonA Szebeni
Feb 19, 2002·EMBO Reports·Giuseppe GianniniAlberto Gulino
May 18, 2004·Trends in Genetics : TIG·Pascale BertrandBernard S Lopez
Jun 16, 2004·Cancer Metastasis Reviews·Andrew R Cuddihy, Robert G Bristow
Jul 29, 2004·DNA Repair·David W Meek
Nov 27, 2004·Cancer Chemotherapy and Pharmacology·Saadia HassanElin Lindhagen
Dec 17, 2004·Nucleic Acids Research·Sheng YunAndrew C G Porter
Jan 24, 2006·Biochemical and Biophysical Research Communications·Lukas M OrreJenny Flygare

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Citations

Aug 13, 2009·Journal of Cell Science·Vivek SrivastavaSagar Sengupta
Oct 20, 2006·International Journal of Radiation Biology·Francesco MarchettiAndrew J Wyrobek
Oct 13, 2006·Journal of Cellular Biochemistry·E Scott Helton, Xinbin Chen
Dec 19, 2016·Chemical Research in Toxicology·Xiufang SongYang Song

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