p53 mutation and cyclin D1 amplification correlate with cisplatin sensitivity in xenografted human squamous cell carcinomas from head and neck

Acta Oncologica
Eva HenrikssonPeter Wahlberg

Abstract

To investigate the response of tumour growth to cisplatin treatment, in relation to p53 mutation and cyclin D1 dysregulation on DNA and protein level, biopsies from seven xenografted human squamous cell carcinomas from the head and neck were analysed with immunohistochemistry for p53 expression and cyclin D1 expression. Polymerase chain reaction-singlestranded conformation polymorphism was used to determine p53 mutations. Fluorescence in situ hybridization was performed to analyse cyclin D1 amplification. The mice were injected i.p. with NaCl (controls) or cisplatin. After injection the tumour volume were measured. The inhibition of tumour growth by cisplatin was defined as the area under the growth curves, and compared with the growth curves of the tumours in the control group. Xenografts with p53 mutation showed significantly higher resistance to cisplatin (p < 0.001) and also tumours with cyclin D1 amplification showed significantly higher resistance (p < 0.001).

References

Jul 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·F CognettiD Giannarelli
Feb 15, 1984·International Journal of Cancer. Journal International Du Cancer·J Wennerberg
Nov 4, 1994·Science·S W LoweT Jacks
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·P W HindsR A Weinberg
Nov 6, 1996·Journal of the National Cancer Institute·W M KochD Sidransky
Mar 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H Raybaud-DiogèneB Têtu
Sep 24, 1999·Hematology/oncology Clinics of North America·J Bourhis, J P Pignon
Mar 29, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A CabelguenneP Laurent-Puig
Jun 19, 2001·Mutation Research·M Kartalou, J M Essigmann

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Citations

Apr 18, 2012·Nature Reviews. Clinical Oncology·John J TentlerS Gail Eckhardt
Jun 23, 2009·Cancer Biotherapy & Radiopharmaceuticals·Maria BjurbergElisabeth Kjellén
Oct 24, 2008·International Journal of Cancer. Journal International Du Cancer·Xiaojian ZhouPing Zhang
Mar 23, 2012·International Journal of Cancer. Journal International Du Cancer·Xiangming JiSmiti V Gupta
Dec 16, 2016·Oral Diseases·P Ramos-GarcíaM A González-Moles
Mar 15, 2011·Breast Cancer Research and Treatment·L KastlA C Schofield
Nov 22, 2012·Experimental and Therapeutic Medicine·Xian-Lu ZhuoYing-Hui Tan
Feb 15, 2020·BMC Cancer·Anas H A Abu-HumaidanOle E Sørensen
Jul 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elisabetta MarangoniMarie-France Poupon

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