p53 mutation profiling of multiple esophageal carcinoma using laser capture microdissection to demonstrate field carcinogenesis

International Journal of Cancer. Journal International Du Cancer
Shuhei ItoYoshihiko Maehara

Abstract

Inactivation of the p53 tumor suppressor gene is one of the most frequent genetic alterations observed in human esophageal carcinomas. In patients with esophageal carcinoma, one of the significant pathological features of the tumor is the presence of multiple lesions within the esophagus. However, the molecular mechanisms involved in the occurrence of multiple lesions have remained elusive. To characterize p53 alterations in multiple esophageal carcinomas and to study their roles in carcinogenesis, we performed p53 immunohistochemical and p53 mutation analyses using laser capture microdissection on surgically resected human esophageal carcinomas from 11 patients: 9 patients with multiple esophageal carcinomas, 1 with an intramural metastasis lesion within the esophagus and 1 with an intraepithelial carcinoma lesion contiguous to the main lesion. In each of the patients with multiple esophageal carcinomas, we examined samples from 1 main lesion and 1 representative concomitant lesion. Molecular analyses of samples from fresh-frozen normal tissues and tumor tissues of the main lesion (whole tumor) were also performed by the same method. p53 protein accumulation was observed in 16 (72.7%) of 22 lesions from the 11 cases. No p53 mu...Continue Reading

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Citations

Apr 19, 2007·Prostate Cancer and Prostatic Diseases·K SircarM Trifiro
Aug 25, 2012·Journal of Biomedicine & Biotechnology·Marilanda Ferreira BelliniAna Elizabete Silva
Aug 31, 2013·Surgery Today·Masaru MoritaYoshihiko Maehara
Nov 23, 2010·Cancer Letters·Chen ChenQianjin Lu
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Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hiroshi SaekiYoshihiko Maehara
May 1, 2021·Cancers·Giovana de Godoy FernandesCarlos Eduardo Fonseca-Alves

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