p59Hck isoform induces F-actin reorganization to form protrusions of the plasma membrane in a Cdc42- and Rac-dependent manner.

The Journal of Biological Chemistry
S CarrénoIsabelle Maridonneau-Parini

Abstract

Hck is a protein kinase of the Src family specifically expressed in phagocytes as two isoforms, p59Hck and p61Hck, localized at the plasma membrane and lysosomes, respectively. Their individual involvement in functions ascribed to Hck, phagocytosis, cell migration, and lysosome mobilization, is still unclarified. To investigate the specific role of p59Hck, a constitutively active variant in fusion with green fluorescent protein (p59Hck(ca)) was expressed in HeLa cells. p59Hck(ca) was found at focal adhesion sites and triggered reorganization of the actin cytoskeleton, leading to plasma membrane protrusions where it co-localized with F-actin. Similarly, microinjection of p59Hck(ca) cDNA in J774.A1 macrophages induced membrane protrusions. Whereas kinase activity and membrane association of p59Hck were dispensable for location at focal adhesions, p59Hck-induced membrane protrusions were dependent on kinase activity, plasma membrane association, and Src homology 2 but not Src homology 3 domain and were inhibited by dominant-negative forms of Cdc42 or Rac but not by blocking Rho activity. A dominant negative form of p59Hck inhibited the Cdc42- and Rac-dependent FcgammaRIIa-mediated phagocytosis. Expression of the Cdc42/Rac-interact...Continue Reading

References

Jan 1, 1977·Scandinavian Journal of Immunology·G Kaplan
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M F MoranT Pawson
Oct 1, 1985·The Journal of Cell Biology·B GeigerT Volberg
Jan 3, 1997·The Journal of Biological Chemistry·H Welch, I Maridonneau-Parini
Feb 13, 1997·Nature·F SicheriJ Kuriyan
Mar 1, 1997·Molecular and Cellular Biology·J HanD Broek
Jan 1, 1997·Annual Review of Immunology·M Daëron
Jul 24, 1997·Biochimica Et Biophysica Acta·I IbarrolaA J Verhoeven
Oct 7, 1997·The Journal of Experimental Medicine·M T CrowleyA L DeFranco
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A Lowell, G Berton
Nov 3, 1998·The EMBO Journal·P MassolP Chavrier
Dec 16, 1998·Experimental Cell Research·K D IzadiD L Durden
Feb 2, 1999·Experimental Cell Research·J Thyberg, S Moskalewski
Feb 9, 2000·The Journal of Experimental Medicine·H D GreshamC L Willman
Feb 24, 2000·The Journal of Experimental Medicine·C J Fitzer-AttasC A Lowell
Mar 14, 2000·The Journal of Biological Chemistry·F DoussauB Poulain
Jun 13, 2000·The Journal of Biological Chemistry·J M LionbergerT E Smithgall
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T SuzukiZ Honda
Sep 1, 2000·The Journal of Biological Chemistry·S CarrénoI Maridonneau-Parini
Oct 12, 2000·Nature Cell Biology·G Chimini, P Chavrier
Feb 13, 2001·Immunology Letters·L CassardC Sautès-Fridman
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S PereiraC Lowell
May 3, 2001·Cellular Immunology·K S Nair, S M Zingde
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·C Wilde, K Aktories
Apr 16, 2002·Molecular Biology of the Cell·Jayesh C PatelEmmanuelle Caron

❮ Previous
Next ❯

Citations

Mar 31, 2005·Trends in Immunology·Giorgio BertonClifford A Lowell
Feb 16, 2012·The Journal of Biological Chemistry·Romain GuietIsabelle Maridonneau-Parini
Jun 4, 2014·Immunology and Cell Biology·Anthony TroegelerGeanncarlo Lugo-Villarino
Jul 7, 2005·Traffic·Céline CougouleIsabelle Maridonneau-Parini
May 5, 2005·The European Journal of Neuroscience·Federica PimpinelliIsabelle Maridonneau-Parini
Oct 17, 2014·Immunological Reviews·Isabelle Maridonneau-Parini
Nov 25, 2004·Experimental Cell Research·Silvia ContinoloGiorgio Berton
Sep 23, 2014·Biochimica Et Biophysica Acta·Roni LevinDaniel Schlam
Dec 31, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Renaud PoinclouxVéronique Le Cabec
Mar 21, 2006·European Journal of Cell Biology·Renaud PoinclouxIsabelle Maridonneau-Parini
May 4, 2007·Experimental Cell Research·Vegesna RadhaGhanshyam Swarup
Dec 12, 2017·Nucleic Acids Research·Alexander J Diaz de ArceClifford L Wang
May 16, 2007·Journal of Cellular Physiology·Renaud PoinclouxVéronique Le Cabec
Oct 11, 2007·Molecular Reproduction and Development·Louis-Jean Bordeleau, Pierre Leclerc
Jan 26, 2006·The Journal of Biological Chemistry·Céline CougouleEmmanuelle Caron
Jun 8, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christel VérolletIsabelle Maridonneau-Parini
May 15, 2007·The Journal of Biological Chemistry·Claire VincentArnaud Labrousse
May 22, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christel VérolletIsabelle Maridonneau-Parini
Feb 1, 2014·The Journal of Biological Chemistry·Haein ParkDianne Cox
May 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yanling LiuGötz R A Ehrhardt
Oct 9, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna BaruzziGiorgio Berton
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Fernando Montaño-RendónGlenn F W Walpole
Nov 14, 2020·Cancer Research·Hisae YoshitomiZigang Dong

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.