p62/p56 are cortical granule proteins that contribute to formation of the cortical granule envelope and play a role in mammalian preimplantation development

Molecular Reproduction and Development
T HoodbhoyP Talbot

Abstract

The purpose of this study was to identify specific cortical granule protein(s) that form the cortical granule envelope and examine their role(s) in fertilization and preimplantation development. The polyclonal antibody A-BL2 was used to show that the cortical granules of mice, rats, hamsters, cows, and pigs contain a pair of proteins designated p62/p56. These proteins are released from hamster cortical granules at fertilization and contribute to formation of the cortical granule envelope, an extracellular matrix present in the perivitelline space of fertilized mammalian oocytes. P62/p56 were present in the cortical granule envelope throughout preimplantation development and were found in blastomere cortices of 4-cell to blastocyst stage embryos. Hamster oocytes fertilized in vivo in the presence of A-BL2 were all monospermic, suggesting that p62/p56 do not function in blocking polyspermy. Likewise treatment of morula to blastocyst stage hamster embryos with A-BL2 had no effect on the implantation of blastocysts. However, cleavage divisions were inhibited in vivo in a dose-dependent manner when fertilized oocytes or 2-cell embryos were treated with A-BL2. Inhibition of cell division was more pronounced in 2-cell embryos than in ...Continue Reading

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Citations

May 11, 2012·Journal of Assisted Reproduction and Genetics·Qiuping PengYanping Kuang
Oct 14, 2005·Biology of Reproduction·Ricardo C RibasIan Wilmut
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Min LiuPrue Talbot
Nov 18, 2011·Reproductive Biology and Endocrinology : RB&E·Min Liu
Jan 9, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·María Dolores SaavedraRaquel Romar
May 12, 2004·Developmental Biology·Levent TutuncuCarmen J Williams
Jun 18, 2003·Microscopy Research and Technique·P Talbot, Pramila Dandekar
Jul 22, 2005·Birth Defects Research. Part C, Embryo Today : Reviews·Richard L Gardner
Jul 31, 2020·International Journal of Molecular Sciences·Yulia N CajasEncina M González
Feb 12, 2008·Acta histochemica·Edward J CarrollSteven B Oppenheimer
Feb 16, 2006·Reproductive Biomedicine Online·R L Gardner

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