P66Shc expression in diabetic rat retina

BMC Ophthalmology
Ming-Hui ZhaoPeirong Lu

Abstract

P66Shc is partially localised within the mitochondrial fraction. It is primarily related to the generation of mitochondrial reactive oxygen species and apoptosis. Based on previous studies, we hypothesize that in the retina, p66Shc may exist and affect the development of diabetic retinopathy. The purpose of this study was to investigate p66Shc expression in retinal in streptozotocin-induced diabetic (SD) rats, which may provide a pathway to study the pathogenesis of diabetic retinopathy. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were used to detect retinal p66Shc mRNA and protein expression in SD rats, respectively. Immunohistochemical staining was applied to detect the location of rat retinal p66Shc expression. TUNEL assay was applied to detect the number of apoptotic cells. P66Shc expression was found in the retina of normal and diabetic rats, and the level of mRNA and protein expression increased with the progression of diabetes mellitus (DM). P66Shc expression was mainly located in the retinal ganglion cell layer and inner nuclear layer. Compared with the normal group, retinal cell tissue apoptosis rate in the D12w group was significantly increased. Rat retinal p66Shc expression was mainly in...Continue Reading

References

Jan 5, 2002·The Journal of Cell Biology·Metello InnocentiGiorgio Scita
Nov 28, 2002·JAMA : the Journal of the American Medical Association·Matthew J Sheetz, George L King
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliPierGiuseppe Pelicci
Jan 30, 2004·Molecular and Cellular Biology·Sonia PaciniCosima T Baldari
Jun 3, 2004·Circulation·Germana ZaccagniniMaurizio C Capogrossi
Jul 31, 2004·Journal of the American Society of Nephrology : JASN·Fiona T H LeeJosephine M Forbes
Oct 27, 2004·Circulation·Pietro FranciaFrancesco Cosentino
Dec 22, 2004·Kidney International·Rangnath MishraMichael S Simonson
Nov 5, 2005·Acta Biochimica Et Biophysica Sinica·Ting-Jun FanJin Liang
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Giovanni G CamiciFrancesco Cosentino
Nov 7, 2009·The Journal of Biological Chemistry·Alexey A TomilovGino A Cortopassi
Feb 17, 2010·Aging·Mirella TrineiMarco Giorgio
Sep 17, 2011·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Andreas Bringmann, Peter Wiedemann
Jan 31, 2012·Cellular Signalling·Paul D RayYoshiaki Tsuji
Jan 1, 2014·Frontiers in Physiology·Ya Fatou Njie-MbyeSunny E Ohia

❮ Previous
Next ❯

Citations

Jun 6, 2019·Basic Research in Cardiology·Kerstin BoenglerRainer Schulz
Mar 3, 2019·BMC Ophthalmology·Ming-Hui ZhaoPeirong Lu
Aug 17, 2020·Ageing Research Reviews·Hilal Ahmad MirFirdous A Khanday
Oct 13, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·ChunKiu HuiGino Cortopassi
Sep 17, 2021·PloS One·Radhia HamdiBachir Benarba

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

PRIMER PREMIER
Statistical Package for Social Sciences
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis