PMID: 8464876Apr 1, 1993Paper

p70 lupus autoantigen binds the enhancer of the T-cell receptor beta-chain gene

Proceedings of the National Academy of Sciences of the United States of America
H MessierA Fotedar

Abstract

The p70 (Ku) autoantigen has been described as a nonhistone nuclear protein recognized by antibodies from lupus patients. In our studies on the regulation of T-cell receptor (TCR) beta-chain gene expression we have identified the p70 lupus autoantigen as a DNA-binding protein that binds the enhancer of the TCR beta-chain gene. This enhancer is essential for expression of the TCR beta gene. The core TCR beta enhancer contains the E3 motif, which we show here is essential for enhancer activity. The protection of the E3 motif in T cells and the marked reduction in enhancer activity when the E3 motif is mutated underline its physiological importance in regulating beta enhancer activity. The p70 lupus autoantigen gene was identified by screening T-cell lambda gt11 libraries with an E3 probe. The gene encodes a protein which binds the E3 motif in a sequence-specific manner. The identification of a 70-kDa protein as a major E3-binding protein by UV crosslinking is consistent with the conclusion that the p70 lupus autoantigen binds the beta enhancer. Finally, we have shown that T-cell nuclear proteins which bind the E3 motif bear p70 (Ku) lupus autoantigenic determinants. Together these data suggest that the p70 autoantigen binds a cri...Continue Reading

References

Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·R Fotedar, J M Roberts
Jul 26, 1985·Biochimica Et Biophysica Acta·M YanevaH Busch
Aug 1, 1989·The Journal of Clinical Investigation·W H ReevesR G Lahita
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt

❮ Previous
Next ❯

Citations

Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M R RobertsF H Ruddle
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·E L BeallD C Rio
Sep 25, 1994·Nucleic Acids Research·M OnoJ D Capra
Feb 17, 2006·Biochimica Et Biophysica Acta·Charles GulloGerrard Teoh
Sep 29, 2011·European Journal of Cell Biology·Ute RaffetsederPeter R Mertens
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Oct 27, 1997·The Journal of Biological Chemistry·O OsipovichK Muegge
Feb 28, 1997·The Journal of Biological Chemistry·T M Bliss, D P Lane
Dec 31, 1997·The Journal of Biological Chemistry·A E OchemA Falaschi
Aug 8, 1998·The Journal of Biological Chemistry·H TorranceR J Haché
Sep 1, 1993·Molecular and Cellular Biology·H MessierA Fotedar

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.