Abstract
Trans-activating (TA) p73 is a member of the p53 family of transcription factors and has been implicated in cell cycle regulation, apoptosis and developmental processes. Although TAp73 positively regulates an overlapping repertoire of genes regulated by p53, TAp73 has been observed to be paradoxically overexpressed in a number of tumor cell types arousing much interest in the post-translational regulation of TAp73 transcriptional activity. Here, we present novel findings that show TAp73 can interact and co-localise, with Polo-Like Kinase 1 (PLK1) and that TAp73 is phosphorylated by this kinase on Threonine-27 (Thr-27) within the TA domain. Using reporter assays and Electrophoretic Mobility Shift Assays (EMSA), our findings suggest that TAp73-mediated activation of the p21(cip/waf), 14-3-3sigma and Bax gene promoters is abrogated by expressed PLK1 for which post-translational modification of TAp73 Thr-27 appears to be a key step in MCF7 cells. Thus highlighting a potential mechanism that uniquely contributes to PLK1-mediated and phosphor-dependent transcriptional deactivation of expressed TAp73.
Citations
Feb 12, 2010·Cellular and Molecular Life Sciences : CMLS·Jung-Eun ParkKyung S Lee
Sep 29, 2011·Clinical & Experimental Metastasis·Yongzhen QianDiane Palmieri
Jul 31, 2010·Nature Reviews. Drug Discovery·Klaus Strebhardt
Jul 8, 2009·Oncogene·A PeschiaroliG Melino
Jan 20, 2016·Antiviral Research·Xiaoyu ChuSongdong Meng
Jul 31, 2014·International Journal of Molecular Sciences·Xiaona JiBuyong Ma
Feb 24, 2016·Biochimica Et Biophysica Acta·Shiv KumarJaebong Kim
Feb 16, 2012·Biochimica Et Biophysica Acta·Kelly D SullivanJoaquín M Espinosa
Nov 26, 2011·Cancer Letters·Hyo-Eun YoonSang-Gun Ahn
Nov 8, 2014·Neoplasia : an International Journal for Oncology Research·Hai LuCarter Van Waes
Nov 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Saifeng WangSongdong Meng
Jan 30, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rossella PellegrinoThomas Longerich
Jun 19, 2013·Cell Reports·Michael WiererMiguel Beato
Jul 16, 2014·International Journal of Genomics·Wei SunBaolin Liu
May 12, 2011·Experimental Biology and Medicine·El Mustapha Bahassi
Jun 18, 2019·The Journal of General Virology·Liyuan QianSongdong Meng
Apr 19, 2017·Oncotarget·Shiv KumarJaebong Kim
Nov 26, 2015·Oncotarget·Martin FischerKurt Engeland
Aug 21, 2013·Oncotarget·Frank Louwen, Juping Yuan
Jun 13, 2019·Blood Advances·Carlos Murga-ZamalloaRyan A Wilcox
Mar 30, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jing LiXingzhi Xu