PAAR proteins act as the 'sorting hat' of the type VI secretion system.

Microbiology
Thomas E WoodAlain Filloux

Abstract

Bacteria exist in polymicrobial environments and compete to prevail in a niche. The type VI secretion system (T6SS) is a nanomachine employed by Gram-negative bacteria to deliver effector proteins into target cells. Consequently, T6SS-positive bacteria produce a wealth of antibacterial effector proteins to promote their survival among a prokaryotic community. These toxins are loaded onto the VgrG-PAAR spike and Hcp tube of the T6SS apparatus and recent work has started to document the specificity of effectors for certain spike components. Pseudomonas aeruginosa encodes several PAAR proteins, whose roles have been poorly investigated. Here we describe a phospholipase family antibacterial effector immunity pair from Pseudomonas aeruginosa and demonstrate that a specific PAAR protein is necessary for the delivery of the effector and its cognate VgrG. Furthermore, the PAAR protein appears to restrict the delivery of other phospholipase effectors that utilise distinct VgrG proteins. We provide further evidence for competition for PAAR protein recruitment to the T6SS apparatus, which determines the identities of the delivered effectors.

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Citations

Nov 14, 2019·Future Microbiology·Biswanath Jana, Dor Salomon
Mar 7, 2020·Environmental Microbiology·Cristian V Crisan, Brian K Hammer
Jun 28, 2020·Cellular Microbiology·Ruth E HernandezSarah J Coulthurst
Jul 14, 2020·Frontiers in Cellular and Infection Microbiology·Sarah WettstadtAlain Filloux
Jul 16, 2020·Pathogens·Luke A F Barretto, Casey C Fowler
Nov 21, 2020·Molecular Microbiology·Dukas Jurėnas, Laure Journet
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Anne-Sophie StolleJohn J Mekalanos
Aug 10, 2021·International Journal of Biological Macromolecules·Haiying WenYuhui Dong
Sep 15, 2021·MBio·Chih-Feng WuJeff H Chang

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Methods Mentioned

BETA
dissection
electrophoresis

Software Mentioned

SignalP
Prism
GraphPad
mega
MAFFT
WebLogo
PAAR

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