PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential

Journal of Cellular and Molecular Medicine
Xiaoling GuoJiansu Chen

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a structurally endogenous peptide with many biological roles. However, little is known about its presence or effects in human adipose-derived stem cells (hADSCs). In this study, the expression of PACAP type I receptor (PAC1R) was first confirmed in hADSCs. Maxadilan, a specific agonist of PAC1R, could increase hADSC proliferation as determined by Cell Counting Kit-8 and cell cycle analysis and promote migration as shown in wound-healing assays. Maxadilan also showed anti-apoptotic activity in hADSCs against serum withdrawal-induced apoptosis based on Annexin V/propidium iodide analysis and mitochondrial membrane potential assays. The anti-apoptotic effects of maxadilan correlated with the down-regulation of Cleaved Caspase 3 and Caspase 9 as well as up-regulation of Bcl-2. The chemical neural differentiation potential could be enhanced by maxadilan as indicated through quantitative PCR, Western blot and cell morphology analysis. Moreover, cytokine neural redifferentiation of hADSCs treated with maxadilan acquired stronger neuron-like functions with higher voltage-dependent tetrodotoxin-sensitive sodium currents, higher outward potassium currents and partial electrica...Continue Reading

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Citations

Dec 7, 2019·Journal of Molecular Neuroscience : MN·Gergő JózsaTamás Juhász
Jul 12, 2019·Journal of Cellular and Molecular Medicine·Yong ChenXiaoling Guo

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Methods Mentioned

BETA
PCR
Protein Assay
flow cytometry
ELISA

Software Mentioned

PatchMaster
ImageJ
GraphPad Prism
MultiCycle
GraphPad
PredictProtein
SWISSMODEL
MATLAB
Igor

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