PACAP-38 positively regulates glycoprotein hormone alpha-gene expression in placental cells

Molecular and Cellular Endocrinology
B J Desai, J M Burrin

Abstract

The human glycoprotein hormone alpha-gene is transcriptionally activated by cAMP in placental cells. We have shown that the novel hypothalamic peptide, pituitary adenylate cyclase activating polypeptide, PACAP-38, significantly stimulates intracellular cAMP levels (12-fold increase; P < 0.001) in JEG-3 choriocarcinoma cells. Regulation of alpha-promoter activity was assessed using both the chloramphenicol acetyl transferase (CAT) and the luciferase (LUC) reporter gene systems. alpha-CAT activity was significantly stimulated by PACAP-38 (4-fold increase; P < 0.05) at 24 h with a similar stimulation being seen with a LUC expression vector. The kinetics of stimulation of the alpha-promoter by PACAP-38 were similar to those seen with 8-Br-cAMP and vasoactive intestinal polypeptide (VIP), a peptide which shares 68% homology with PACAP-38. PACAP-38 also stimulated the production of IL-6 from JEG-3 cells with a time course of response similar to that of alpha-promoter transcription. We conclude that human placental choriocarcinoma cells possess functional receptors for PACAP-38, whose activation enhances cAMP formation, alpha-subunit gene transcription and interleukin-6 (IL-6) production.

References

Jan 6, 1992·Biochimica Et Biophysica Acta·M HosoyaA Arimura
Jan 1, 1990·Endocrinology·B L SpangeloP C Isakson
Jul 1, 1989·Endocrinology·B L SpangeloR M MacLeod
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F PetragliaW Vale
Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Aug 1, 1982·The Journal of Clinical Endocrinology and Metabolism·T ShibasakiN Ling
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C M GormanB H Howard
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Jan 18, 1980·Science·G S Khodr, T M Siler-Khodr
Jul 4, 2012·Bioorganic & Medicinal Chemistry Letters·Zaihui ZhangJay A Cadieux

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Citations

Jul 9, 2008·Journal of Molecular Neuroscience : MN·D ReglodiZ Helyes
Jul 8, 2000·Parasite Immunology·R D GillespieR G Titus
Jul 16, 2003·Hypertension·Bernhard M W SchmidtRoland E Schmieder
Feb 21, 2007·Hypertension·David W Good

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