PACAP intraperitoneal treatment suppresses appetite and food intake via PAC1 receptor in mice by inhibiting ghrelin and increasing GLP-1 and leptin

American Journal of Physiology. Gastrointestinal and Liver Physiology
John P VuPatrizia M Germano

Abstract

Pituitary adenylate cyclase-activating peptide (PACAP) is expressed within the gastroenteric system, where it has profound physiological effects. PACAP was shown to regulate food intake and thermogenesis centrally; however, PACAP peripheral regulation of appetite and feeding behavior is unknown. Therefore, we studied PACAP's effect on appetite and food intake control by analyzing feeding behavior and metabolic hormones in PAC1-deficient (PAC1-/-) and age-matched wild-type (WT) mice intraperitoneally injected with PACAP1-38 or PACAP1-27 before the dark phase of feeding. Food intake and feeding behavior were analyzed using the BioDAQ system. Active ghrelin, glucagon-like peptide-1 (GLP-1), leptin, peptide YY, pancreatic polypeptide, and insulin were measured following PACAP1-38 administration in fasted WT mice. PACAP1-38/PACAP1-27 injected into WT mice significantly decreased in a dose-dependent manner cumulative food intake and reduced bout and meal feeding parameters. Conversely, PACAP1-38 injected into PAC1-/- mice failed to significantly change food intake. Importantly, PACAP1-38 reduced plasma levels of active ghrelin compared with vehicle in WT mice. In PAC1-/- mice, fasting levels of active ghrelin, GLP-1, insulin, and lep...Continue Reading

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Citations

May 18, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Takeshi IwasaMinoru Irahara
Jul 4, 2018·Alcoholism, Clinical and Experimental Research·Anuranita GuptaJessica R Barson
Feb 23, 2017·Frontiers in Endocrinology·Revathi SekarBilly Kwok Chong Chow
Jul 10, 2019·Current Topics in Medicinal Chemistry·Chenyi LiaoJianing Li
Jul 5, 2019·Journal of Cellular and Molecular Medicine·Xing XiaoYi Ma
Mar 13, 2021·Trends in Endocrinology and Metabolism : TEM·Léa MontégutGuido Kroemer
Apr 4, 2021·International Journal of Molecular Sciences·Cassandra GastelumEdward J Wagner
Jun 21, 2020·Molecular and Cellular Endocrinology·Stephen J Winters, Joseph P Moore

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