PACAP Promotes Matrix-Driven Adhesion of Cultured Adult Murine Neural Progenitors

ASN Neuro
James A WaschekPawel Niewiadomski

Abstract

New neurons are born throughout the life of mammals in germinal zones of the brain known as neurogenic niches: the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus. These niches contain a subpopulation of cells known as adult neural progenitor cells (aNPCs), which self-renew and give rise to new neurons and glia. aNPCs are regulated by many factors present in the niche, including the extracellular matrix (ECM). We show that the neuropeptide PACAP (pituitary adenylate cyclase-activating polypeptide) affects subventricular zone-derived aNPCs by increasing their surface adhesion. Gene array and reconstitution assays indicate that this effect can be attributed to the regulation of ECM components and ECM-modifying enzymes in aNPCs by PACAP. Our work suggests that PACAP regulates a bidirectional interaction between the aNPCs and their niche: PACAP modifies ECM production and remodeling, in turn the ECM regulates progenitor cell adherence. We speculate that PACAP may in this manner help restrict adult neural progenitors to the stem cell niche in vivo, with potential significance for aNPC function in physiological and pathological states.

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Citations

Aug 26, 2018·Cancers·Jomnarong LertsuwanJutamaad Satayavivad

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Datasets Mentioned

BETA
GSE66193
E-MEXP-322
GSE4496

Methods Mentioned

BETA
Assay
flow cytometry

Software Mentioned

GraphPad Prism
RDAVIDWebService
ImageJ
Affymetrix Expression Console suite
DAVID tool ( Database for Annotation , Visualization , and Int...
DAVID
Excel
Ingenuity Pathway Analysis
R

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