Packaging motor from double-stranded RNA bacteriophage phi12 acts as an obligatory passive conduit during transcription

Nucleic Acids Research
Denis E KainovRoman Tuma

Abstract

Double-stranded RNA viruses sequester their genomes within a protein shell, called the polymerase complex. Translocation of ssRNA into (packaging) and out (transcription) of the polymerase complex are essential steps in the life cycle of the dsRNA bacteriophages of the Cystoviridae family (phi6-phi14). Both processes require a viral molecular motor P4, an NTPase, which bears structural and functional similarities to hexameric helicases. In effect, switching between the packaging and the transcription mode requires the translocation direction of the P4 motor to reverse. However, the mechanism of the reversal remains elusive. Here we characterize the P4 protein from bacteriophage phi12 and exploit its purine nucleotide specificity to delineate P4 role in transcription. The results indicate that while P4 actively translocates RNA during packaging it acts as a passive conduit for RNA export. The directionality switching is accomplished via the regulation of P4 NTPase activity within the polymerase core.

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Citations

Oct 17, 2007·Nature Reviews. Microbiology·Armen Y MulkidjanianEugene V Koonin
Apr 19, 2005·Nature Structural & Molecular Biology·Jirí LísalRoman Tuma
Oct 4, 2013·Journal of Virology·Xiaoyu SunMinna M Poranen
Jul 24, 2008·Journal of Molecular Biology·Ertan EryilmazRanajeet Ghose
Nov 11, 2006·Journal of Structural Biology·Juha T HuiskonenSarah J Butcher
Nov 3, 2016·RNA·Kalle J HanhijärviMinna M Poranen
Dec 7, 2007·The Journal of Biological Chemistry·Denis E KainovRoman Tuma
Mar 4, 2006·Neurosurgical Focus·Todd McCallDouglas Brockmeyer
Feb 15, 2020·PLoS Computational Biology·Jordan DouglasAlexei J Drummond
Apr 21, 2005·The Journal of Biological Chemistry·Jirí Lísal, Roman Tuma

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