PMID: 9525584Apr 3, 1998Paper

Packaging of endogenous retroviral sequences in retroviral vectors produced by murine and human packaging cells

Journal of Virology
C PatienceR A Weiss

Abstract

Interaction of retrovirus vectors and endogenous retroviruses present in packaging cell lines and target cells may result in unwanted events, such as the formation of recombinant viruses and the mobilization of therapeutic vectors. Using sensitive reverse transcriptase PCR assays, we investigated human and murine gene therapy packaging cell lines for incorporation of endogenous retrovirus transcripts into murine leukemia virus (MLV) vector particles and, conversely, whether vector genomes are incorporated into human endogenous retrovirus (HERV) particles. VL30 endogenous retrovirus sequences were efficiently packaged in particles produced by the murine AM12 packaging system. For every seven MLV-derived beta-galactosidase (beta-Gal) vector genomes present in the particles, one copy of VL30 was also packaged. Although human FLY packaging cells expressed several classes of HERV transcripts (HERV-K, HuRT, type C, and RTVL-H), none was detectable in the MLV vector particles released from the cells. Nonspecific packaging of the MLV Gag-Pol expression vector transcripts was detected in the FLY virions at a low level (1 in 17,000 sequences). These findings indicate that human packaging cells produce retrovirus particles far less contam...Continue Reading

References

Oct 1, 1992·The Journal of Experimental Medicine·R E DonahueR Moen
Oct 1, 1992·Human Gene Therapy·F L Moolten, L A Cupples
May 1, 1990·Virology·M A Sommerfelt, R A Weiss
Jan 1, 1990·Human Gene Therapy·K CornettaW F Anderson
Jan 1, 1990·Journal of Virology·D T ScaddenJ M Cunningham
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R A AndersonR D Camerini-Otero
Mar 1, 1997·Nature Medicine·C PatienceR A Weiss
Mar 1, 1997·Trends in Genetics : TIG·C PatienceR A Weiss

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Citations

Apr 8, 2000·Journal of Biomedical Science·J G Mikkelsen, F S Pedersen
May 23, 2012·Fundamental & Clinical Pharmacology·Bernard JoverJean-Pierre Molès
Feb 27, 2001·Journal of Virology·C PatienceR A Weiss
Mar 12, 2002·Journal of Cardiac Surgery·J A Fishman
Jul 6, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yuri MartinaDaniel R Salomon
Oct 5, 2006·Retrovirology·Robin A Weiss
May 10, 2013·Current Opinion in Rheumatology·Matthew F CusickRobert S Fujinami
Dec 3, 2014·Human Gene Therapy·A Dusty Miller
Aug 23, 2016·Viruses·Alice Telesnitsky, Sandra L Wolin
Jan 4, 2003·Blood·Christopher BaumChristof von Kalle
Jul 12, 2002·The Journal of Gene Medicine·Miguel Sena-EstevesXandra O Breakefield
May 18, 2000·Reviews in Medical Virology·G PalùM Pizzato
Aug 11, 2000·Acta Tropica·D K Langat, J M Mwenda

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