Packed-column capillary electrochromatography and capillary electrochromatography-mass spectrometry using a lithocholic acid stationary phase.

Electrophoresis
Dean Norton, Shahab A Shamsi

Abstract

The preparation and characterization of a novel lithocholic acid (LCA)-based liquid crystalline (LC) stationary phase (SP) suitable for application in packed-column CEC and CEC coupled to MS is described. The extent of bonding reactions of LCA-SP was assessed using 1H-NMR, 13C-NMR and elemental analysis. This characterization is followed by application of the LCA-SP for separation of beta-blockers, phenylethylamines (PEAs), polyaromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). Using the optimum mobile phase operating conditions (pH 3.0-4.5, 10 mM ammonium acetate, 85% v/v ACN), a comparison of the chromatographic ability of the aminopropyl silica phase vs. the LCA-bonded phase was conducted. The results showed improved selectivity for all test analytes using the latter phase. For example, the CEC-MS of beta-blockers demonstrated that the LCA-bonded phase provides separation of six out of seven beta-blockers, whereas the amino silica phase provides four peaks of several co-eluting beta-blockers. For the CEC-MS analysis of PEAs, the LCA-bonded phase showed improved resolution and different selectivity as compared to the aminopropyl phase. An evaluation of the retention trends for PEAs on both phases suggested tha...Continue Reading

References

Jul 16, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·B BuszewskiS Kowalska
Feb 26, 2004·Electrophoresis·Dean Norton, Shahab A Shamsi
Jun 3, 2004·Electrophoresis·Joseph J PesekMilton T W Hearn
Jul 9, 2004·Electrophoresis·Elena Barceló-BarrachinaM Teresa Galceran
Sep 10, 2004·Journal of Chromatography. a·Christian W Klampfl
Oct 6, 2004·Journal of Chromatography. a·G Brent DawsonRichard R Seipert
Feb 1, 2005·Journal of Chromatography. a·Zygfryd WitkiewiczMarta Repelewicz
Nov 1, 2005·Analytical Chemistry·Dean NortonShahab A Shamsi

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Citations

Jun 17, 2010·Se pu = Chinese journal of chromatography·Zian LinGuonan Chen
Jul 21, 2010·Journal of Separation Science·Laura MercoliniMaria Augusta Raggi

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