PMID: 8956329Nov 1, 1996Paper

Paclitaxel and nocodazole differentially alter endocytosis in cultured cells

Pharmaceutical Research
S F Hamm-AlvarezW C Shen

Abstract

Microtubule-based transport facilitates the endocytosis of exogenous macromolecules. We have determined how microtubule accumulation and disassembly alter endocytosis. The effects of paclitaxel, which promotes microtubule assembly, and nocodazole, which promotes microtubule disassembly, on fluid-phase and receptor-mediated endocytosis were measured using uptake of horseradish peroxidase and 125I-transferrin, respectively. Changes in membrane and microtubule organization were examined by fluorescence microscopy. Neither paclitaxel (4 microM, 60 min pretreatment) nor nocodazole (1 microgram/ml, 60 min pretreatment) significantly inhibited fluid-phase endocytosis. However, paclitaxel caused a redistribution of fluorescent fluid-phase marker to the periphery. Both paclitaxel and nocodazole treatment significantly (p < or = 0.05) reduced the initial uptake of 125I-transferrin at 5 min to approximately 50% of control. Despite the similarity of the effects on initial endocytic uptake, the effects on steady state accumulation of 125I-transferrin were quite distinct. Exposure of CV-1 cells to paclitaxel for an additional 30, 60 or 90 min also showed reduced accumulation of 125I-transferrin up to a maximum significant (p < or = 0.05) inh...Continue Reading

Citations

Dec 14, 2018·Cells·Ping Wee, Zhixiang Wang
May 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hongtao ChenJi-Xin Cheng
Jan 9, 2020·Anti-cancer Drugs·Stephanie Shishido, Thu Annelise Nguyen
Sep 19, 2007·Journal of Biomedical Materials Research. Part a·Wen-Jen Lin, Kai-Ling Chang
Nov 24, 1999·Human Gene Therapy·M BrissonL Huang
Jul 19, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M CasartelliB Giordana
Apr 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Puja Sapra, Theresa M Allen
Jan 12, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Shariq AliErik Rytting
Jun 6, 2000·Advanced Drug Delivery Reviews·S F Hamm-Alvarez
Sep 5, 2003·European Journal of Pharmacology·Marta MichalikWłodzimierz Korohoda
May 30, 2021·Developmental Cell·Tien Phan-EversonEric D Siggia
Nov 5, 2003·Advanced Drug Delivery Reviews·John W Murray, Allan W Wolkoff

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