Paclitaxel Priming of TRAIL Expressing Mesenchymal Stromal Cells (MSCs-TRAIL) Increases Antitumor Efficacy of Their Secretome

Current Cancer Drug Targets
Valentina CoccèAugusto Pessina

Abstract

Adipose tissue derived MSCs engineered with the tumor necrosis factor-related apoptosis-inducing ligand protein (MSCs-TRAIL) have a significant anticancer activity. MSCs, without any genetic modifications, exposed to high doses of chemotherapeutic agents are able to uptake the drug and release it in amount affecting tumor proliferation. The purpose of this study was to verify the ability of MSCs-TRAIL to uptake and release paclitaxel (PTX) by providing an increased antitumor efficacy. MSCs and MSCs-TRAIL were tested for their sensitivity to Paclitaxel (PTX) by MTT assay and the cells were loaded with PTX according to a standardized procedure. The secretome was analysed by HPLC for the presence of PTX, microarray assay for soluble TRAIL (s-TRAIL) and tested for in vitro anticancer activity. MSCs-TRAIL were resistant to PTX and able to incorporate and then release the drug. The secretion of s-TRAIL by PTX loaded MSCs-TRAIL was not inhibited and the PTX delivery together with s-TRAIL secretion resulted into an increased antitumor efficacy of cell secretoma as tested in vitro on human pancreatic carcinoma (CFPAC-1) and glioblastoma (U87-MG). Our result is the first demonstration of the possible merging of two new MSCs therapy appro...Continue Reading

References

Jul 1, 1977·Journal of the National Cancer Institute·J FoghT Orfeo
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A SchoumacherT A Rado
Oct 1, 1988·The American Journal of Medicine·J C McIntoshR E Tiller
Dec 20, 2003·Current Molecular Medicine·Juan ShiRuian Xu
Mar 11, 2008·Cancer Letters·Frank A E Kruyt
Nov 26, 2008·Circulation Research·Massimiliano GnecchiVictor J Dzau
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laura S SasportasKhalid Shah
Mar 20, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Caren E Petrie Aronin, Rocky S Tuan
Jul 12, 2011·Advanced Drug Delivery Reviews·Khalid Shah
Aug 2, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Luigi BianconeGiovanni Camussi
Mar 15, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayF Bray
Apr 12, 2013·Stem Cells International·Devang M PatelAnand S Srivastava
Sep 21, 2013·International Journal of Immunopathology and Pharmacology·A BonomiA Pessina
Oct 1, 2013·Trends in Molecular Medicine·Daniel W Stuckey, Khalid Shah
Dec 26, 2013·Stem Cells and Development·Bin ZhangSai Kiang Lim
Jun 20, 2014·Anti-cancer Agents in Medicinal Chemistry·Massimo MariottiAugusto Pessina
Aug 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luisa PascucciAugusto Pessina
Mar 17, 2015·Methods : a Companion to Methods in Enzymology·Ji Sun ParkKam W Leong
Mar 18, 2016·Expert Opinion on Drug Delivery·Anna Teresa BriniGiampietro Farronato
Jul 28, 2016·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
Oct 18, 2016·Cytotherapy·Elizabeth K SageSam M Janes
Feb 20, 2017·Cellular and Molecular Life Sciences : CMLS·Na Li, Jinlian Hua
Jul 25, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Barbara CrivelliUNKNOWN Italian Mesenchymal Stem Cell Group (GISM)
Feb 8, 2018·Translational Oncology·Jones GyamfiJunjeong Choi
Sep 8, 2018·PloS One·Maria Giovanna ScioliAugusto Orlandi
Sep 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Isabella RimoldiAugusto Pessina
Feb 12, 2019·Scientific Reports·Carlotta SpanoMassimo Dominici

❮ Previous
Next ❯

Citations

Jul 30, 2021·Frontiers in Cell and Developmental Biology·Ali HassanzadehMostafa Jarahian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis