Paclitaxel promotes lung cancer cell apoptosis via MEG3-P53 pathway activation

Biochemical and Biophysical Research Communications
Jianhao XuYusong Zhang

Abstract

Paclitaxel (PTX) is a first-line chemotherapy drug for advanced non-small cell lung cancer (NSCLC). The long-chain non-coding RNA maternally expressed gene 3 (MEG3) is a recognized tumor suppressor. This study aimed to explore the effects of PTX on the expression of MEG3 and its anti-tumor mechanism in lung cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed to determine cell proliferation. Quantitative polymerase chain reaction was used to determine the levels of MEG3 expressions. Western blot and immunofluorescence were used to detect protein levels. Small interfering RNA or pCDNA-MEG3 transfection was used to downregulate or upregulate MEG3 expression. Dichlorof luorescein diacetate was used to detect intracellular reactive oxygen species. Flow cytometry was used to analyze apoptosis. PTX significantly inhibited the proliferation of NSCLC cells and increased the expressions of MEG3 and P53. The downregulation of MEG3 attenuated PTX-induced cytotoxicity, whereas upregulation of MEG3 induced cell death and increased P53 expression. The inhibition of P53 caused no effect on the upstream MEG3 expression. Our results suggest that the MEG3-P53 pathway is involved in the apoptosis of A54...Continue Reading

Citations

May 23, 2020·International Journal of Molecular Sciences·Christophe PouletJulien Guiot
Feb 15, 2020·International Journal of Molecular Sciences·Xing-Ning LaiLi-Xia Xiong
Sep 25, 2020·Asia-Pacific Journal of Clinical Oncology·Rana Muhammad UsmanFaiz Anwer
Oct 3, 2020·Bioengineering & Translational Medicine·Louis J BornSteven M Jay
Dec 4, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zhenyao ChenZhaoxia Wang
Mar 25, 2021·Cell Biology International·Surendar AravindhanMajid Ahmadi

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