Paclitaxel resistance in MCF-7/PTX cells is reversed by paeonol through suppression of the SET/phosphatidylinositol 3-kinase/Akt pathway

Molecular Medicine Reports
Weipeng ZhangYalin Dong

Abstract

Breast cancer is one of the most prevalent types of malignant tumor. Paclitaxel is widely used in the treatment of breast cancer; however, the major problem contributing to the failure of chemotherapy in breast cancer is the development of drug resistance. Therefore, it is necessary to identify novel therapeutic targets and reversal agents for breast cancer. In the present study, the protein expression levels of SET, protein phosphatase 2A (PP2A) and phosphatidylinositol 3-kinase (PI3K)/Akt pathway were determined in MCF-7/PTX human breast carcinoma paclitaxel-resistant cells using western blot analysis. Small interference RNAs (siRNAs) were used to knock down the gene expression of SET in MCF-7/PTX cells and the cell viability was assessed following treatment with paclitaxel, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assays and flow cytometry. In addition, western blot analysis was used to determined PI3K/Akt pathway activity following SET knockdown. Furthermore, the reversal effects of paeonol on paclitaxel, and its underlying mechanisms of action, were investigated using western blot analysis and reverse transcription-quantitative polymerase chain reaction. The results demonstrated that increased le...Continue Reading

References

Oct 31, 2002·The Journal of Biological Chemistry·Núria CanelaOriol Bachs
Aug 3, 2004·The Journal of Pharmacy and Pharmacology·Li-ming ChenLi-wu Fu
Sep 16, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MadeiraB Allinquant
Jul 25, 2006·The Journal of Biological Chemistry·Sabine WagnerUta-Maria Bauer
Aug 25, 2006·Toxicology and Applied Pharmacology·Kazuhiro IshiguroHidemi Goto
Mar 26, 2008·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H HuangH H Kim
May 2, 2008·Archives of Pharmacal Research·Bomi LeeDong-Hyun Kim
Mar 14, 2009·The Journal of Biological Chemistry·Rajat Bhattacharya, Fernando Cabral
Feb 17, 2010·Current Drug Discovery Technologies·Mahmoud YounsThomas Efferth
Oct 9, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Nirmala Bhoo-PathyHelena M Verkooijen
Feb 22, 2013·Molecular Cancer Therapeutics·Sivapriya PonnurangamDharmalingam Subramaniam
Jun 19, 2013·Acta histochemica·Juan YinHuan Yang
Jul 19, 2013·International Journal of Endocrinology·Xu BoqunXiang Ma
Oct 31, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Hyojung LeeHyunsu Bae
Nov 5, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zonglei MaoXiaoqiang Dong
Nov 5, 2013·Biochemical and Biophysical Research Communications·Zhaoming LiMingzhi Zhang
Jan 30, 2014·Breast Cancer Research : BCR·Armina A KaziAngela H Brodie
Dec 3, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jiangxia CaiYalin Dong

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Citations

Jun 20, 2018·The Journal of Pharmacology and Experimental Therapeutics·Chao-Yuan HuangKuen-Feng Chen
Nov 12, 2016·Journal of Physiology and Biochemistry·Yi XuYan-Yue Tong
Jun 29, 2017·Scientific Reports·Satoru KakeKoichi Sato
Mar 27, 2019·Scientific Reports·Nehal GuptaSanjay K Srivastava
Aug 6, 2020·Marine Drugs·Reda F A AbdelhameedUsama Ramadan Abdelmohsen
Jan 12, 2021·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Luciana MoscaGianni Colotti
Jun 15, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Shu-Yu YangMeng-Jen Lee
Oct 7, 2021·Drug Development and Industrial Pharmacy·Qian ZhangYuewu Wang

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