Oct 20, 1989

PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes

E LarsenBarbara C Furie


PADGEM (platelet activation dependent granule-external membrane protein) is an integral membrane protein of the alpha granules of platelets and Weibel-Palade bodies of endothelial cells that is expressed on the plasma membrane upon cell activation and granule secretion. Activated platelets, but not resting platelets, bind to neutrophils, monocytes, HL60 cells, and U937 cells. This interaction is inhibited by anti-PADGEM antibodies, PADGEM, and EDTA; anti-GPIIb-IIIa, anti-thrombospondin, anti-GPIV, and thrombospondin produce no effect. Neutrophils and U937 cells, in contrast to Jurkatt cells, contain PADGEM recognition sites, as shown by binding of PADGEM contained in phospholipid vesicles. These results indicate that PADGEM mediates adhesion of activated platelets to monocytes and neutrophils. Therefore, PADGEM shares not only structural but also functional homology with ELAM-1 and MEL-14, members of a new family of vascular cell adhesion molecules.

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Mentioned in this Paper

Monoclonal Antibodies
SELP gene
Weibel-Palade Bodies
Neutrophil Band Cells
Blood Vessel
Platelet Adhesiveness
Neutrophils as Percentage of Blood Leukocytes (Lab Test)
Platelet Membrane Glycoproteins
CD36 wt Allele

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