PADI4‑mediated epithelial‑mesenchymal transition in lung cancer cells

Molecular Medicine Reports
Meiyan LiuLi Cai

Abstract

Lung cancer is a complex disease involving multiple genetic and phenotypic alterations. As a histone modification enzyme, protein‑arginine deiminase type‑4 (PADI4) and its downstream signaling have been studied in the progression of a variety of types of human cancer, but data on PADI4‑mediated posttranslational modification in lung cancer are lacking. The aim of present study was to evaluate the expression of PADI4 and its associated molecular signaling in lung cancer metastasis. The results of the present study indicated that PADI4 was overexpressed in lung cancer cells, while knockdown of PADI4 could lead to attenuation of the lung cancer cell invasion and migration phenotype, which was further verified by determining the epithelial‑mesenchymal transition (EMT) marker proteins. Additionally, it was demonstrated that stable knockdown of PADI4 in A549 lung cancer cells resulted in a striking reduction of the EMT‑associated Snail1/mothers against decapentaplegic homolog 3/4 transcriptional complex, which was consistent with alterations in migratory and invasive phenotypes of A549 lung cancer cells. Therefore, PADI4‑mediated EMT transition is proposed to represent a novel mechanism underlying the epigenetic and phenotypic altera...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Mar 13, 2010·Cancer Cell International·Xiaotian Chang, Kehua Fang
Sep 10, 2010·International Journal of Biological Sciences·Lin WangPengcheng Wang
Feb 16, 2012·Nature Communications·Chizu TanikawaKoichi Matsuda
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sonja C StadlerC David Allis
Jan 28, 2014·Nature·Maria A ChristophorouTony Kouzarides
May 31, 2014·Nature Communications·Stephan KolodziejJörn Lausen
Mar 26, 2015·World Journal of Gastroenterology : WJG·Yun-Fei XuJie Yang
May 13, 2015·Cancer Metastasis Reviews·Ding ZhangXiangdong Wang
Jul 8, 2015·The Lancet Oncology·Siddhartha DevarakondaRamaswamy Govindan
Nov 25, 2016·Experimental and Therapeutic Medicine·Jiang Xin, Xiuqi Song
Dec 15, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Weixin LiangXiaoyu Gu
Apr 5, 2017·Anticancer Research·Nikolaos TsoukalasStamatios Theocharis
May 31, 2018·Cell Death & Disease·Tae Woo KimSeong-Gyu Ko

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Citations

Jul 8, 2020·International Journal of Molecular Sciences·Elif Damla ArisanSigrun Lange
Oct 5, 2021·Frontiers in Oncology·Pei DaiYurong Tan

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad
Graphpad Prism
Odyssey

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