Abstract
Background: Retinal photoreceptor (RP) cells are widely involved in retina-related diseases, and oxidative stress plays a critical role in retinal secondary damage. Herein, we investigated the effectiveness and potential mechanisms of autophagy of paeonol (Pae) in terms of oxidation resistance. Methods: The animal model was induced by light damage (LD) in vivo, whereas the in vitro model was established by H2O2 stimulation. The effectiveness of Pae was evaluated by hematoxylin and eosin, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, immunofluorescence, transmission electron microscopy, electroretinogram, and Western blot analysis in vivo, and the underlying mechanisms of Pae were assessed by Cell Counting Kit-8 assay, reactive oxygen species (ROS) assay, and Western blot analysis in 661W cells. We mainly evaluated the effects of Pae on apoptosis and autophagy. Results: Increased apoptosis of the LD-induced and decreased autophagy of RPs were mitigated by Pae treatment. Pea, which increased the expression of mitochondrial functional protein cytochrome c, reversed the decreased cell viability and autophagy induced by oxidative stress in 661W cells. Experiments showed that autophagy was downregulated in PINK1...Continue Reading
References
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Elaine TanMuayyad R Al-Ubaidi
Nov 29, 2007·Progress in Retinal and Eye Research·A E Weymouth, A J Vingrys
Dec 1, 2007·Brain Research·Jonathan StoneJan Provis
May 24, 2008·The Journal of Clinical Investigation·Fiona PickfordTony Wyss-Coray
Oct 10, 2009·Cellular and Molecular Neurobiology·Xu ZhuHongzhuan Chen
Jan 1, 2010·Investigative Ophthalmology & Visual Science·Youde Jiang, Jena J Steinle
Mar 8, 2011·Brain Research·Jun ZhouStephen C Bondy
Apr 15, 2011·Cell Death & Disease·R RussoM T Corasaniti
Apr 24, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bendong ChenGuangshun Yang
Jul 6, 2013·Progress in Neurobiology·Antero SalminenMikko Hiltunen
Aug 3, 2013·Journal of Cell Science·Åsa Birna BirgisdottirTerje Johansen
Apr 1, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jiangxia CaiYalin Dong
Aug 13, 2015·Nature·Michael LazarouRichard J Youle
Sep 25, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas R Sundermeier, Krzysztof Palczewski
Nov 19, 2015·Journal of Neuroinflammation·Rebecca ScholzThomas Langmann
Feb 24, 2016·Autophagy·Daniel J Klionsky
Aug 4, 2016·Molecular Medicine Reports·Xiaojin ShiHong-Dang Qu
Aug 28, 2016·Progress in Retinal and Eye Research·Patricia BoyaBeatriz Villarejo-Zori
Apr 4, 2017·Mitochondrion·Evy LefevereInge Van Hove
Mar 8, 2018·International Journal of Molecular Medicine·Yufeng LiZhongming Zhang
Mar 23, 2018·Experimental and Therapeutic Medicine·Bing ZhaoHao Wang
Apr 24, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ker Woon ChoyMohd Rais Mustafa
Aug 14, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Duraisamy RamachandhiranSukumar Babukumar
Feb 23, 2019·Autophagy·Thomas G McWilliamsIan G Ganley
Jul 31, 2019·The American Journal of Pathology·Takashi MiyaiUla V Jurkunas
Dec 8, 2019·Free Radical Biology & Medicine·Jun KimSe-Young Choung
Apr 20, 2020·Free Radical Biology & Medicine·Stefania D'AdamoRosa Maria Borzì