Abstract
Paeonol from Cortex Moutan root is a potential therapeutic agent for atherosclerosis (AS). However, its mechanisms of action are still not fully understood. Vascular endothelial cells (VECs) autophagy plays a vital role in the initiation and progression of AS. In this study, we aim to investigate whether the protective effect of paeonol on ox-LDL-induced VECs injury by regulating autophagy. To address this question, we used ox-LDL-induced rat VECs as a model system to elucidate the protective effect of paeonol on VECs injury. This study displayed that ox-LDL (100 mg/L) treatment inhibited VEC growth in dose- and time-dependent manners, paeonol (60 μM) shown potential in inhibiting ox-LDL-induced death. Furthermore, paeonol significantly reduced ox-LDL-induced the formation of autophagy vacuoles and the expression of LC3II in VECs. Further double-luciferase reporter assay shown that miR-30a specifically binds to the 3'-UTR of Beclin-1 mRNA in VECs. Moreover, we found that ox-LDL decreased miR-30a and increased Beclin-1 expression, pretreatment with paeonol could reverse the process of regulation in dose-dependent manners. In ox-LDL treated VECs, transfection with a miR-30a mimic significantly increased miR-30a expression and inh...Continue Reading
References
Jan 1, 1995·Annual Review of Physiology·R Ross
Jun 7, 2003·Histology and Histopathology·Y-P ChauM-H Tai
Nov 6, 2004·Science·Takahiro Shintani, Daniel J Klionsky
Oct 4, 2005·The Journal of Clinical Investigation·Beth Levine, Junying Yuan
Jul 24, 2007·European Journal of Cell Biology·Marcin NowickiKatharina Spanel-Borowski
Mar 26, 2008·Autophagy·Li YuMichael J Lenardo
Nov 13, 2008·Planta medica·Houkai LiWei Jia
Jun 19, 2009·Autophagy·Hua ZhuJin-Ming Yang
Nov 6, 2010·Journal of Cardiovascular Pharmacology·Shanshan Qin, Chunxiang Zhang
Mar 29, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Pin-Kuei FuChing-Liang Hsieh
Jan 30, 2013·Journal of Ethnopharmacology·Mei-Hua BaoHong-Hao Zhou
Jun 19, 2013·Journal of Innate Immunity·Daniel J Klionsky, Patrice Codogno
Nov 19, 2013·BMC Neurology·Guangwen LongDao Wen Wang
Feb 25, 2014·Acta Pharmacologica Sinica·Ya-rong LiuMin Dai
Apr 1, 2014·Cell·Douglas R Green, Beth Levine
Jan 27, 2015·Oncogene·S Fulda, D Kögel
Jan 31, 2015·Circulation Research·Damián GaticaDaniel J Klionsky
Mar 7, 2015·The Journal of Toxicological Sciences·Masanori OchiHiromu Toyoda
Nov 19, 2015·Journal of Cardiovascular Pharmacology·Yun LuoXiao-Bo Sun
Feb 3, 2016·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Kai WuJunshan Zhai
Oct 21, 2016·Clinical and Experimental Pharmacology & Physiology·Fan Jiang
Oct 18, 2016·International Journal of Molecular Medicine·Xusheng YuanMin Dai
Dec 19, 2016·Trends in Endocrinology and Metabolism : TEM·Makon-Sébastien Njock, Jason E Fish
Feb 18, 2017·Circulation Research·Ulrich FörstermannHuige Li
Mar 24, 2017·Current Opinion in Lipidology·Binod AryalCarlos Fernández-Hernando
Citations
Oct 26, 2018·Stem Cell Research & Therapy·Daohai QianXiaoming Wang
Oct 28, 2019·Epigenomics·Tianen ZhouYanran Liang
Dec 6, 2018·Frontiers in Pharmacology·Yarong LiuMin Dai
Dec 14, 2018·Oxidative Medicine and Cellular Longevity·Li LuXiaomei Guo
Dec 6, 2019·Scientific Reports·Xiaoxiao ZhangYonghong Hu
Aug 7, 2019·International Journal of Molecular Sciences·Li Yang, Jian Pan
Aug 10, 2020·Molecular and Cellular Biochemistry·Yunfu YuJifeng Yan
Feb 9, 2021·Frontiers in Pharmacology·Chin-Feng TsaiShiang-Suo Huang
Oct 24, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Qing LiuHong-Feng Gu
May 21, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Patrick Kwabena OduroQilong Wang
Jun 12, 2021·PeerJ·Qian ZhangChunxiang Zhou
Nov 7, 2020·Life Sciences·Yarong LiuMin Dai
Aug 10, 2021·Frontiers in Cardiovascular Medicine·Min WuLongtao Liu
Aug 18, 2021·Autoimmunity·Guan-Xiu TangShang-Ping Zhao
Sep 16, 2021·Critical Reviews in Clinical Laboratory Sciences·Anvarsadat KianmehrAbdolkarim Mahrooz