PAI-1 transcriptional regulation during the G0 --> G1 transition in human epidermal keratinocytes

Journal of Cellular Biochemistry
Li QiPaul J Higgins

Abstract

Plasminogen activator inhibitor type-1 (PAI-1) is the major negative regulator of the plasmin-dependent pericellular proteolytic cascade. PAI-1 gene expression is normally growth state regulated but frequently elevated in chronic fibroproliferative and neoplastic diseases affecting both stromal restructuring and cellular migratory activities. Kinetic modeling of cell cycle transit in synchronized human keratinocytes (HaCaT cells) indicated that PAI-1 transcription occurred early after serum stimulation of quiescent (G0) cells and prior to entry into a cycling G1 condition. PAI-1 repression (in G0) was associated with upstream stimulatory factor-1 (USF-1) occupancy of two consensus E box motifs (5'-CACGTG-3') at the PE1 and PE2 domains in the PF1 region (nucleotides -794 to -532) of the PAI-1 promoter. Chromatin immunoprecipitation (ChIP) analysis established that the PE1 and PE2 site E boxes were occupied by USF-1 in quiescent cells and by USF-2 in serum-activated, PAI-1-expressing keratinocytes. This reciprocal and growth state-dependent residence of USF family members (USF-1 vs. USF-2) at PE1/PE2 region chromatin characterized the G0 --> G1 transition period and the transcriptional status of the PAI-1 gene. A consensus E box ...Continue Reading

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Citations

Apr 4, 2008·Archives of Dermatological Research·Kirwin M ProvidencePaul J Higgins
May 28, 2013·Cell Structure and Function·Shu TanakaYoshihiro Yoneda
Jul 16, 2010·The Journal of Investigative Dermatology·Takuya TakeichiYasushi Tomita
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z MaC K Oh
Apr 30, 2010·The Journal of Investigative Dermatology·Jennifer FreytagPaul J Higgins
Oct 16, 2012·Cellular Signalling·Rohan SamarakoonPaul J Higgins
Mar 17, 2016·The Journal of Endocrinology·Erika GalgocziEndre V Nagy
Sep 6, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alisha RamosYoon Pin Lim
Jul 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Craig E HigginsPaul J Higgins
Jul 5, 2011·Journal of Clinical Pathology·Jung-Woo ChoiYoung-Sik Kim

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