Paired cysteine residues are required for high levels of the Helicobacter pylori autotransporter VacA

Microbiology
Darren P LetleyJohn C Atherton

Abstract

The Helicobacter pylori vacuolating cytotoxin VacA shares homology in its C-terminal domain with many autotransporter proteins, suggesting a similar mechanism of secretion. Like most autotransporters, VacA contains a single pair of cysteine residues located near the C-terminus of the passenger domain. This study aimed to investigate the role of these conserved cysteine residues. This involved changing each cysteine in the VacA passenger domain to serine, quantifying the effect on VacA levels and assessing toxin activity in H. pylori. It was shown that both cysteine residues were required for high VacA levels, although mutation of each cysteine reduced toxin amounts to differing extents, implying that their importance was not simply for intramolecular disulphide bond formation. Although less VacA was observed for the cysteine mutants, vacuolating activity was detected, showing that the cysteines were not required for VacA function.

References

Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
Apr 1, 1988·Journal of Bacteriology·A Labigne-RousselL Tompkins
Jun 1, 1988·Journal of Medical Microbiology·R D LeunkD R Morgan
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·E PapiniC Montecucco
May 5, 1994·The New England Journal of Medicine·J ParsonnetG D Friedman
May 1, 1994·The Journal of Experimental Medicine·J L TelfordZ Xiang
Jan 1, 1993·Molecular Microbiology·E PapiniC Montecucco
Dec 29, 1995·The Journal of Biological Chemistry·T SuzukiC Sasakawa
Aug 1, 1996·Journal of Medical Microbiology·E PapiniC Montecucco
Oct 6, 1997·The Journal of Biological Chemistry·M MolinariC Montecucco
Jul 1, 1997·Molecular Membrane Biology·B J Loveless, M H Saier
Oct 21, 1998·Trends in Microbiology·I R HendersonJ P Nataro
Dec 6, 2000·The Journal of Experimental Medicine·K OguraM Omata
Dec 19, 2000·Infection and Immunity·V Q NguyenT L Cover
Jan 4, 2003·Journal of Bacteriology·David C OliverRachel C Fernandez
May 10, 2003·The Journal of Biological Chemistry·Darren P LetleyJohn C Atherton
Oct 22, 2003·Molecular Immunology·Nicola FitchenKim R Hardie
Jun 11, 2005·Journal of Medical Microbiology·Nicola FitchenKim R Hardie

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Citations

Oct 19, 2011·The Journal of Biological Chemistry·Denisse L LeytonIan R Henderson
Oct 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kelly A GangwerD Borden Lacy
Jan 15, 2014·Medical Molecular Morphology·Hong WuKouichi Sano
Feb 19, 2008·Trends in Microbiology·Xaver SewaldRainer Haas
Oct 16, 2007·Trends in Microbiology·Harris D Bernstein
Feb 17, 2007·Molecular Microbiology·Wouter S P JongJoen Luirink
Sep 16, 2006·Chembiochem : a European Journal of Chemical Biology·Darryl FalzaranoHeinz Feldmann
Sep 22, 2015·Frontiers in Microbiology·Kathryn P Haley, Jennifer A Gaddy
Jul 17, 2013·Critical Reviews in Microbiology·Toon NicolayStijn Spaepen
Jan 10, 2012·Journal of Molecular Biology·Ana SauríJoen Luirink
Apr 24, 2016·Toxicon : Official Journal of the International Society on Toxinology·Muhammad JunaidGerd Katzenmeier
Apr 15, 2017·Applied Microbiology and Biotechnology·Katarzyna M Bocian-OstrzyckaElżbieta Katarzyna Jagusztyn-Krynicka

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