Pairing of heterochromatin in response to cellular stress

Experimental Cell Research
H I Abdel-HalimJ J W A Boei

Abstract

We previously reported that exposure of human cells to DNA-damaging agents (X-rays and mitomycin C (MMC)) induces pairing of the homologous paracentromeric heterochromatin of chromosome 9 (9q12-13). Here, we show that UV irradiation and also heat shock treatment of human cells lead to similar effects. Since the various agents induce very different types and frequencies of damage to cellular constituents, the data suggest a general stress response as the underlying mechanism. Moreover, local UV irradiation experiments revealed that pairing of heterochromatin is an event that can be triggered without induction of DNA damage in the heterochromatic sequences. The repair deficient xeroderma pigmentosum cells (group F) previously shown to fail pairing after MMC displayed elevated pairing after heat shock treatment but not after UV exposure. Taken together, the present results indicate that pairing of heterochromatin following exposure to DNA-damaging agents is initiated by a general stress response and that the sensing of stress or the maintenance of the paired status of the heterochromatin might be dependent on DNA repair.

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Citations

Nov 28, 2007·Nucleic Acids Research·Rut ValgardsdottirGiuseppe Biamonti
Jun 13, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Choong-Ryoul SihnSang Hoon Kim
Nov 28, 2007·Nature Reviews. Genetics·Adi Barzel, Martin Kupiec

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