Pall leukotrap affinity prion-reduction filter removes exogenous infectious prions and endogenous infectivity from red cell concentrates

Vox Sanguinis
Samuel O Sowemimo-CokerRichard Carp

Abstract

Three recent probable cases of transmission of a variant of human Creutzfeldt-Jakob disease (vCJD) through blood transfusion suggest that the disease can be transmitted through transfusion of blood components from presymptomatic blood donors. In this study, we investigated the performance of a new filter for reducing the levels of infectious prions (PrP(Sc)) from red cell concentrates (RCC). Endogenous Infectivity: A pool of 500 ml of whole blood was collected from 263K-strain scrapie-infected hamsters into an anticoagulant, processed into non-leucoreduced RCC (NL-RCC), and then passed through a prion-reduction filter. Pre- and postfiltration samples were tested for PrP(Sc) by Western blot and infectivity by inoculation of healthy hamsters. Results of the endogenous infectivity study after 200 days post-inoculation are discussed. Exogenous (Spiking) Study: Scrapie-infected hamster brain homogenates containing PrP(Sc) were added to human RCC and then filtered. Levels of PrP(Sc) were determined by Western blot assay. The effect of prior leucodepletion of 'spiked' RCC on PrP(Sc) removal by the prion-removal filter was also assessed. In the endogenous infectivity study, at 200-day observation time, the prefiltered RCC transmitted d...Continue Reading

References

Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P E BendheimS B Prusiner
Sep 1, 1980·The Journal of Clinical Investigation·N MohandasS B Shohet
Feb 1, 1983·British Journal of Haematology·R T CardP L Mollison
Jan 23, 1993·Lancet·T F EsmondeW B Matthews
Apr 6, 1996·Lancet·R G WillP G Smith
Jul 31, 1998·Transfusion·S O Sowemimo-CokerB Wenz
Oct 21, 2000·Lancet·F HoustonC J Bostock
Sep 10, 2002·Biologicals : Journal of the International Association of Biological Standardization·M VeyA Gröner
Oct 22, 2002·The Journal of General Virology·Nora HunterFiona Houston
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J W Ironside, M W Head
Aug 11, 2004·Lancet·Alexander H PedenJames W Ironside

❮ Previous
Next ❯

Citations

Jan 3, 2007·Seminars in Hematology·Harvey J AlterRoger Y Dodd
Jan 11, 2011·Journal of Toxicology and Environmental Health. Part a·Michael G TyshenkoDaniel Krewski
Aug 19, 2011·BMC Clinical Pathology·Daisuke OkuzakiHiroshi Nojima
Sep 28, 2007·Current Opinion in Hematology·Roger Y Dodd
Aug 10, 2013·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S ThomasL M Williamson
Jun 2, 2010·Clinics in Laboratory Medicine·Roger Y Dodd
May 15, 2007·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Jean-Jacques Lefrère
Dec 26, 2006·Lancet·Marc L Turner
Feb 18, 2009·Vox Sanguinis·J CosteUNKNOWN Subgroup on TSE

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bovine Spongiform Encephalopathy

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.

Bovine Spongiform Encephalopathy (MDS)

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.