Palmatine, a protoberberine alkaloid, inhibits both Ca(2+)- and cAMP-activated Cl(-) secretion in isolated rat distal colon.

British Journal of Pharmacology
D Z WuZ B Hu

Abstract

The protoberberine alkaloid berberine has been reported to inhibit colonic Cl(-) secretion. However, it is not known if other protoberberine alkaloids share these effects. We have therefore selected another protoberberine alkaloid, palmatine, to assess its effects on active ion transport across rat colonic epithelium. Rat colonic mucosa was mounted in Ussing chambers and short circuit current (I (SC)), apical Cl(-) current and basolateral K(+) current were recorded. Intracellular cAMP content was determined by an enzyme immunoassay. Intracellular Ca(2+) concentration was measured with Fura-2 AM. Palmatine inhibited carbachol-induced Ca(2+)-activated Cl(-) secretion and the carbachol-induced increase of intracellular Ca(2+) concentration. Palmatine also inhibited cAMP-activated Cl(-) secretion induced by prostaglandin E(2) (PGE(2)) or forskolin. Palmatine prevented the elevation of intracellular cAMP by forskolin. Determination of apical Cl(-) currents showed that palmatine suppressed the forskolin-stimulated, apical cAMP-activated Cl(-) current but not the carbachol-stimulated apical Ca(2+)-activated Cl(-) current. Following permeabilization of apical membranes with nystatin, we found that palmatine inhibited a carbachol-stimul...Continue Reading

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Citations

Sep 4, 2014·The American Journal of Chinese Medicine·Chunqiu ChenMartin Storr
Dec 23, 2015·Chemistry : a European Journal·Jayachandran Jayakumar, Chien-Hong Cheng
Feb 23, 2019·Journal of Separation Science·Jiahui WangNing Zhang
Jan 16, 2010·Medicinal Research Reviews·Kakali Bhadra, Gopinatha Suresh Kumar
Apr 15, 2020·Minerva gastroenterologica e dietologica·Francesco Di PierroLeandro Ivaldi

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