Palmitate-induced changes in protein expression of insulin secreting INS-1E cells

Journal of Proteomics
Meri HovsepyanPeter Bergsten

Abstract

Elevated blood levels of glucose and lipids in individuals with type 2 diabetes mellitus have been observed to cause impairment of insulin secretion from pancreatic beta-cells. Chronic exposure to either of the circulating fatty acid oleate or palmitate has different effects on the beta-cell. Whereas palmitate causes functional impairment of the beta-cell and apoptosis, oleate has only minor negative effects on beta-cell function and mass. The aim of the present study was to delineate mechanisms by which the fatty acids affect the beta-cell differently. In particular, the aim was to identify beta-cell proteins exclusively regulated by palmitate. INS-1E cells were cultured for 24h in medium supplemented with palmitate or oleate. After culture, cells were lysed and subjected to two-dimensional gel electrophoresis. Proteins specifically regulated by palmitate were excised from the gel and identified by peptide mass fingerprinting using MALDI-TOF MS. Proteins exclusively regulated by palmitate were classified into proteins of carbohydrate or protein metabolism and Ca(2+) or mRNA binding proteins. The specific palmitate-induced down-regulation of enzymes of glycolysis, proteins of protein turnover and anti-apoptotic protein may cont...Continue Reading

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Citations

Jan 12, 2013·Analytical and Bioanalytical Chemistry·Sarojini J K A UbhayasekeraJonas Bergquist
Nov 26, 2011·Lipids in Health and Disease·Juliana MagdalonRui Curi
Oct 6, 2010·Lipids in Health and Disease·Kristofer ThörnPeter Bergsten
Nov 23, 2012·Journal of Proteome Research·Michael MarisLut Overbergh
Mar 3, 2011·Proteomics·Wannes D'HertogChantal Mathieu
Aug 24, 2018·BMC Genomics·Ernest SargsyanPeter Bergsten
Aug 8, 2018·Molecular & Cellular Proteomics : MCP·Zonghong LiJunjie Hou
Nov 1, 2018·Molecular & Cellular Proteomics : MCP·Zonghong LiJunjie Hou
Jul 3, 2021·Frontiers in Physiology·Maria Eugenia Vázquez-MosqueraFrancisco J Blanco

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