Palmitate induced lipoapoptosis of exocrine pancreas AR42J cells

Apoptosis : an International Journal on Programmed Cell Death
Z LandauR Z Birk

Abstract

Chronic surplus of dietary consumption, typical to obesity, results in overflow of fat to non-adipose tissues. Intracellular accumulation of fat in non-adipose tissues is associated with cellular dysfunction and cell death and ultimately contributes to the pathogenesis of chronic diseases. The influence of fat overflow on the exocrine pancreas is not known. The purpose of this research was to study the lipotoxic and lipoapoptotic effect of prolonged (72 h) long chain saturated palmitic fatty acid (0.1 mM) on the survival of exocrine pancreas AR42J cells. We demonstrate that chronic exposure of AR42J cells to palmitic acid results in significant increase in triglycerides accumulation (up to 25% of cells area), compared to untreated cultures. Lipid accumulation prompted a typical apoptotic process, demonstrated by both DNA fragmentation and condensed chromatin appearance (DAPI staining). Quantitative real-time PCR studies demonstrated that prolonged palmitic acid supplementation induced down-regulation of the anti-apoptotic Bcl2 mRNA levels (22%) and up-regulation of the pro-apoptotic Bax mRNA levels (300%), leading to disruption of the pro/anti apoptotic balance (Bax/Bcl2=3). No major change was detected in iNOS mRNA expression....Continue Reading

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Citations

Nov 17, 2012·Expert Review of Molecular Diagnostics·Carla Ferreri, Chryssostomos Chatgilialoglu
Jan 1, 2008·Nutrition·Anna AronisOren Tirosh
Oct 4, 2015·Experimental Cell Research·Hila DaninoRuth Birk
Aug 31, 2014·Experimental Cell Research·Angela S AndersonEva M Schmelz
Mar 11, 2008·Biochimica Et Biophysica Acta·Choon Pei Low, Hongyuan Yang
Apr 15, 2010·Biochimica Et Biophysica Acta·Eduardo RialM Mar González-Barroso
Feb 27, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Joung Hoon AhnHyeok Yil Kwon
Feb 17, 2019·International Journal of Molecular Sciences·Roberta ScanferlatoCarla Ferreri

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