Palmitate-induced PTP1B expression is mediated by ceramide-JNK and nuclear factor κB (NF-κB) activation

Cellular Signalling
Narges MohammadTaghvaeiReza Meshkani

Abstract

Palmitate induces PTP1B expression in skeletal muscle cells. The purpose of this study was to investigate the mechanisms responsible for palmitate-induced PTP1B expression in mouse skeletal muscle cells. Three truncated fragments of PTP1B promoter were cloned into PGL3-basic vector and the promoter activity of PTP1B was assessed in C2C12 cells exposed to palmitate either in the presence or in the absence of several inhibitors to study the biochemical pathways involved. EMSA was performed to examine binding of NF-κB to NF-κB consensus sequence and PTP1B oligonucelotides in the cells treated with palmitate. Lentiviral PTP1B-shRNA was used to knockdown PTP1B in myotubes. The phosphorylation and protein levels of IRS-1 and Akt were detected by western blot. 0.5mM palmitate induced PTP1B promoter activity in fragment -1715/+59 by 50% (p<0.01). Palmitate increased NF-κB binding to both NF-κB consensus sequence and one NF-κB sequence (-920 to -935) in PTP1B promoter. Incubation of C2C12 cells with different concentrations of C2-ceramide enhanced PTP1B promoter activity dose-dependently. Inhibitors of de novo ceramide synthesis prevented palmitate-induced PTP1B promoter activity in myotubes. In addition, inhibitor of JNK pathway preven...Continue Reading

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Citations

Dec 12, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Ahmad NasimianReza Meshkani
Aug 18, 2012·The Journal of Endocrinology·Sattar Gorgani-FiruzjaeeReza Meshkani
Dec 9, 2015·Archives of Physiology and Biochemistry·Elisa LucasRocio Vila-Bedmar
Sep 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Hadi KhodabandehlooReza Meshkani
Feb 9, 2016·Journal of Agricultural and Food Chemistry·Yuanyuan JingGang Shu
Jun 14, 2014·Biochemical and Biophysical Research Communications·Sattar Gorgani-FiruzjaeeReza Meshkani
Oct 19, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Reza Meshkani, Sanaz Vakili
Mar 12, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H B LiW J Jiang
Jul 22, 2017·Journal of Applied Physiology·Ismael Perez-SuarezJose A L Calbet
Aug 3, 2018·Journal of Molecular Endocrinology·Louise K MetcalfeNigel Turner
May 20, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Jia FengYi Ma
Aug 14, 2019·Nutrition Journal·Maria E Morgan-Bathke, Michael D Jensen
May 23, 2017·Frontiers in Endocrinology·Lewan ParkerItamar Levinger
Jun 19, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Camila Lopez-CrisostoSergio Lavandero

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