Palmitoyl ascorbate and doxorubicin co-encapsulated liposome for synergistic anticancer therapy

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Yue YangNing Li

Abstract

Combination therapy with two drugs and nanoparticle-based drug delivery systems are widely applied to reduce the adverse effects of traditional treatment by chemotherapeutic drugs. Palmitoyl ascorbate (PA) as a lipophilic derivative of ascorbic acid shows the advantages in cancer treatment. The aim of the study was to prepare a doxorubicin (DOX) and PA co-loaded liposome to synergistically treat tumor and effectively alleviate the toxicity caused by DOX. The effects were evaluated by in vitro and in vivo studies. The liposomes (weight ratio of DOX to PA=1:20, DOX1/PA20-LPs) exhibited the strongest synergistic effects, combination index was 0.38, 0.56, and 0.05 in MCF-7, HepG2, and A549 cells, respectively. In vitro cellular uptake study, the intercellular concentration of DOX in DOX1/PA20-LPs was 2.5-fold greater than DOX loaded liposome, and DOX1/PA20-LPs was taken in not only by macropinocytosis, but also by clathrin-mediated endocytosis. Intracellular distribution experiment showed that DOX1/PA20-LPs efficiently concentrated in the nucleus. In vivo studies indicated that co-encapsulated liposome not only showed the strongest antitumor ability by tumor growth suppression, but also significantly enhanced the safety by the chan...Continue Reading

Citations

Aug 25, 2019·Drug Delivery and Translational Research·Xiaoyu LuNing Li
Jul 19, 2018·Frontiers in Physiology·Margreet C M Vissers, Andrew B Das
Nov 5, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Amanda Costa CaritáGislaine Ricci Leonardi
Aug 25, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jingwen FuTianfu Wu

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