Palmitoyl-CoA stimulates cellular uptake and plasma membrane binding of carnitine

Biochemical and Biophysical Research Communications
B Gustafson, L A Ransnäs

Abstract

Carnitine cellular uptake and plasma membrane binding was investigated in S49 lymphoma cells. Palmitoyl-CoA was found to increase membrane binding of carnitine from 506 +/- 48 to 8,690 +/- 235 pmol/mg membrane protein. Palmitate and CoA acted synergistically and increased carnitine binding to plasma membranes but could not replace palmitoyl-CoA. The effect of palmitoyl-CoA on membrane binding of carnitine was maximal at 10 microM and required the presence of ATP. Palmitoyl-CoA increased the cellular uptake rate of carnitine from 181 +/- 5 to 884 +/- 25 amol/cell and h-1. We conclude that palmitoyl-CoA is a major regulator of cellular uptake of carnitine and, based on quantitative estimations, that the carnitine carrier binds more than one carnitine molecule.

References

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Feb 13, 1997·Biochemical and Biophysical Research Communications·B Gustafson, L A Ransnäs

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Citations

Mar 21, 2001·Biochimica Et Biophysica Acta·R R RamsayF R van der Leij

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