Palmitoylation of CD36/FAT regulates the rate of its post-transcriptional processing in the endoplasmic reticulum

Biochimica Et Biophysica Acta
Rick F ThorneGordon F Burns

Abstract

CD36/FAT is a transmembrane glycoprotein that functions in the cellular uptake of long-chain fatty acids and also as a scavenger receptor. As such it plays an important role in lipid homeostasis and, pathophysiologically, in the progression of type 2 diabetes and atherosclerosis. CD36 expression is tightly regulated at the levels of both transcription and translation. Here we show that its expression and location are also regulated post-translationally, by palmitoylation. Although palmitoylation of CD36 was not required for receptor maturation and cell surface expression, inhibition of palmitoylation either pharmacologically with cerulenin or by mutation of the relevant cysteines delayed processing at the ER and trafficking through the secretory pathway. The absence of palmitoylation also reduced the half life of the CD36 protein. Additionally, the CD36 palmitoylation mutant did not incorporate efficiently into lipid rafts, a site known to be required for its function of fatty acid uptake, and this reduced the efficiency of uptake of oxidized low density lipoprotein. These findings provide an added level of sophistication where translocation of CD36 to the plasma membrane may be physiologically regulated by palmitoylation.

References

Jun 1, 1990·The Journal of Experimental Medicine·B A BiggsJ H Leech
Jul 1, 1994·The Journal of Cell Biology·A M Shenoy-ScariaD M Lublin
Feb 15, 1995·Biochemical and Biophysical Research Communications·F A Van NieuwenhovenJ F Glatz
Jan 19, 1996·The Journal of Biological Chemistry·M AlessioR Sitia
Sep 13, 1996·The Journal of Biological Chemistry·N TaoD M Lublin
Dec 17, 1997·Biochemical and Biophysical Research Communications·R F ThorneP G Gibson
Jun 26, 1999·The Journal of Biological Chemistry·M FebbraioR L Silverstein
May 9, 2000·The Journal of Biological Chemistry·A BonenN N Tandon
Aug 31, 2000·Biochemical and Biophysical Research Communications·P GruarinM Alessio
May 2, 2001·The Journal of Cell Biology·B J NicholsJ Lippincott-Schwartz
Sep 19, 2001·The Journal of Clinical Investigation·M FebbraioR L Silverstein
Jan 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Joost J F P LuikenArend Bonen
Mar 2, 2002·Journal of Diabetes and Its Complications·Nobutaka OhgamiHitoshi Nakayama
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Ronit Simantov, Roy L Silverstein
Sep 23, 2003·The Biochemical Journal·Gonzalo L Vilas, Luc G Berthiaume
Oct 1, 2003·The Journal of Cell Biology·Laurent Gagnoux-PalaciosFilippo G Giancotti
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fran BalamuthKim Bottomly
Sep 29, 2004·Journal of Virology·Rebecca B Katzman, Richard Longnecker
Oct 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Maria FebbraioRoy L Silverstein
Oct 22, 2004·Molecular Biology of the Cell·Jürgen PohlWolfgang Stremmel
Jan 26, 2005·Biochemical Society Transactions·V A Drover, N A Abumrad
Feb 15, 2005·Biochemical and Biophysical Research Communications·Bernhard SchmidBradford S Hamilton
Mar 9, 2005·The Journal of Biological Chemistry·Hidehiko ShogomoriDeborah A Brown
Mar 17, 2005·Journal of Cell Science·Christina M Van ItallieJames M Anderson
Jun 24, 2005·The EMBO Journal·Javier Valdez-Taubas, Hugh Pelham

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Citations

Sep 9, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew LynesEric P Widmaier
May 24, 2014·Metabolism: Clinical and Experimental·Wen-Jun ShenSalman Azhar
Nov 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gregory J FredericksPeter R Hoffmann
Apr 5, 2013·The FEBS Journal·Sanja BlaskovicF Gisou van der Goot
Jul 22, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yufeng ZhangDavid L Swanson
Jun 23, 2011·Dermato-endocrinology·Denis KhnykinFrode Jahnsen
Aug 4, 2016·Frontiers in Endocrinology·Irena A RebalkaThomas J Hawke
Sep 13, 2016·Biochimica Et Biophysica Acta·Joost J F P LuikenJan F C Glatz
Nov 24, 2017·Frontiers in Physiology·Gianfranca CartaClaudia Manca
Sep 19, 2017·Nature Reviews. Nephrology·Xiaochun YangXiong Z Ruan
Mar 14, 2018·British Medical Bulletin·Lei ZhaoXiong Z Ruan
Jul 13, 2018·Critical Reviews in Biochemistry and Molecular Biology·María-Eugenia Zaballa, F Gisou van der Goot
Jun 13, 2019·Biological Trace Element Research·Michael P Marciel, Peter R Hoffmann
Jun 18, 2011·The Journal of Biological Chemistry·Elham SadeqzadehRick F Thorne
Dec 19, 2019·Cells·Hong-Tai TzengWei-Yu Chen
Jun 9, 2018·International Journal of Molecular Sciences·Loïze MaréchalAndré Tremblay
Mar 31, 2019·Nutrients·Zhibo GaiZhenguo Wang
Mar 27, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melanie A MarkovicPatricia L Brubaker
Apr 30, 2020·Diabetes & Metabolism Journal·Jun Sung MoonKyu Chang Won
Feb 20, 2020·The Journal of Biological Chemistry·Alexander L TichoWaddah A Alrefai
Nov 20, 2020·Cardiovascular Research·Hongyang ShuDao Wen Wang
Jan 29, 2021·The FEBS Journal·Hening Lin
Dec 17, 2020·International Journal of Molecular Sciences·Francesco SchianchiJoost J F P Luiken

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