Palmitoylation of proteolipid protein from rat brain myelin using endogenously generated 18O-fatty acids.

The Journal of Biological Chemistry
S U Tetzloff, O A Bizzozero

Abstract

Proteolipid protein (PLP), the major protein of central nervous system myelin, contains covalently bound fatty acids, predominantly palmitic acid. This study adapts a stable isotope technique (Kuwae, T., Schmid, P. C., Johnson, S. B., and Schmid, H. O. (1990) J. Biol. Chem. 265, 5002-5007) to quantitatively determine the minimal proportion of PLP molecules which undergo palmitoylation. In these experiments, brain white matter slices from 20-day-old rats were incubated for up to 6 h in a physiological buffer containing 50% H218O. The uptake of 18O into the carbonyl groups of fatty acids derived from PLP, phospholipids, and the free fatty acid pool was measured by gas-liquid chromatography/mass spectrometry of the respective methyl esters. Palmitic acid derived from PLP acquired increasing amounts of 18O, ending with 2.9% 18O enrichment after 6 h of incubation. 18O incorporation into myelin free palmitic acid also increased over the course of the incubation (67.2% 18O enrichment). After correcting for the specific activity of the 18O-enriched free palmitic acid pool, 7.6% of the PLP molecules were found to acquire palmitic acid in 6 h. This value is not only too large to be the result of the palmitoylation of newly synthesized PL...Continue Reading

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Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier
Jul 9, 2013·Analytical Chemistry·Joshua J NicklayRichard M Caprioli
Nov 18, 2005·Molecular and Cellular Neurosciences·Jörn SchweitzerHauke Werner
Mar 29, 2001·Physiological Reviews·N Baumann, D Pham-Dinh
Jul 30, 2005·Mass Spectrometry Reviews·Susumu Ando, Yasukazu Tanaka

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