Palmitoylation of stathmin family proteins domain A controls Golgi versus mitochondrial subcellular targeting

Biology of the Cell
Stéphanie ChauvinA Sobel

Abstract

Precise localization of proteins to specialized subcellular domains is fundamental for proper neuronal development and function. The neural microtubule-regulatory phosphoproteins of the stathmin family are such proteins whose specific functions are controlled by subcellular localization. Whereas stathmin is cytosolic, SCG10, SCLIP and RB3/RB3'/RB3'' are localized to the Golgi and vesicle-like structures along neurites and at growth cones. We examined the molecular determinants involved in the regulation of this specific subcellular localization in hippocampal neurons in culture. We show that their conserved N-terminal domain A carrying two palmitoylation sites is dominant over the others for Golgi and vesicle-like localization. Using palmitoylation-deficient GFP (green fluorescent protein) fusion mutants, we demonstrate that domains A of stathmin proteins have the particular ability to control protein targeting to either Golgi or mitochondria, depending on their palmitoylation. This regulation involves the co-operation of two subdomains within domain A, and seems also to be under the control of its SLD (stathmin-like domain) extension. Our results unravel that, in specific biological conditions, palmitoylation of stathmin prote...Continue Reading

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Citations

Feb 11, 2010·The Journal of Biological Chemistry·Sylvie LachkarSylvie Ozon
Oct 31, 2012·The Journal of Cell Biology·Bhuvaneish Thangaraj SelvarajMichael Sendtner
Dec 19, 2009·Trends in Endocrinology and Metabolism : TEM·Eva Tomas, Joel F Habener
Aug 8, 2009·Molecular and Cellular Neurosciences·Fabienne E Poulain, André Sobel
Jun 10, 2016·Molecular Neurodegeneration·Sarah BellouzeGeorg Haase
Jun 14, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Eunsil Cho, Mikyoung Park
Dec 20, 2014·PloS One·Sylvie Gory-FauréAnnie Andrieux
Feb 20, 2010·Nature Reviews. Neuroscience·Yuko Fukata, Masaki Fukata
Nov 12, 2009·The Journal of Biological Chemistry·Alonso M HigueroTeresa Iglesias
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Malika BsibsiJohannes M van Noort

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