Palmitoylation of tetraspanin proteins: modulation of CD151 lateral interactions, subcellular distribution, and integrin-dependent cell morphology

Molecular Biology of the Cell
Xiuwei H YangM E Hemler

Abstract

Here we demonstrate that multiple tetraspanin (transmembrane 4 superfamily) proteins are palmitoylated, in either the Golgi or a post-Golgi compartment. Using CD151 as a model tetraspanin, we identified and mutated intracellular N-terminal and C-terminal cysteine palmitoylation sites. Simultaneous mutations of C11, C15, C242, and C243 (each to serine) eliminated >90% of CD151 palmitoylation. Notably, palmitoylation had minimal influence on the density of tetraspanin protein complexes, did not promote tetraspanin localization into detergent-resistant microdomains, and was not required for CD151-alpha 3 beta 1 integrin association. However, the CD151 tetra mutant showed markedly diminished associations with other cell surface proteins, including other transmembrane 4 superfamily proteins (CD9, CD63). Thus, palmitoylation may be critical for assembly of the large network of cell surface tetraspanin-protein interactions, sometimes called the "tetraspanin web." Also, compared with wild-type CD151, the tetra mutant was much more diffusely distributed and showed markedly diminished stability during biosynthesis. Finally, expression of the tetra-CD151 mutant profoundly altered alpha 3 integrin-deficient kidney epithelial cells, such th...Continue Reading

References

Dec 1, 1994·Immunology Today·M D Wright, M G Tomlinson
Jul 28, 1995·The Journal of Biological Chemistry·F BerditchevskiM E Hemler
Sep 4, 1995·International Journal of Cancer. Journal International Du Cancer·K J RadfordP Hersey
Oct 1, 1993·The Journal of Experimental Medicine·A K MatsumotoD T Fearon
Oct 1, 1994·Cell Adhesion and Communication·J M BergelsonR W Finberg
Jun 7, 1996·Biochimica Et Biophysica Acta·M E HemlerF Berditchevski
Apr 4, 1998·Molecular Biology of the Cell·S Gonzalo, M E Linder
Jul 29, 1998·Histochemistry and Cell Biology·A Dinter, E G Berger
Aug 26, 1998·Biochimica Et Biophysica Acta·S C ToddS Levy
Oct 30, 1998·Science·P PileriS Abrignani
Nov 18, 1998·Current Opinion in Cell Biology·M E Hemler
Dec 5, 1998·Biochimica Et Biophysica Acta·J T Dunphy, M E Linder
May 26, 1999·Experimental Hematology·Y TanioS I Hayashi
Jul 31, 1999·The Journal of Cell Biology·F Berditchevski, E Odintsova
Jan 5, 2000·European Journal of Pharmacology·H JinB F O'Dowd
Jan 15, 2000·Science·F Le NaourC Boucheix
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y Yashiro-OhtaniH Fujiwara
Mar 17, 2000·Biochimica Et Biophysica Acta·V SerruE Rubinstein
Mar 29, 2000·The Journal of Biological Chemistry·R L YauchM E Hemler
Sep 14, 2000·Current Biology : CB·E OdintsovaF Berditchevski
Nov 23, 2000·The Journal of Biological Chemistry·C S StippM E Hemler

❮ Previous
Next ❯

Citations

Feb 9, 2012·Cellular and Molecular Life Sciences : CMLS·Chandan SharmaMartin E Hemler
Mar 19, 2013·The International Journal of Biochemistry & Cell Biology·H M SchröderT Ludwig
Apr 23, 2003·Biochemical and Biophysical Research Communications·Stéphanie CharrinEric Rubinstein
Apr 12, 2003·Cellular Signalling·Mónica Yunta, Pedro A Lazo
Dec 14, 2002·Trends in Cell Biology·Marie-José Bijlmakers, Mark Marsh
Feb 11, 2003·Trends in Biochemical Sciences·Christopher S StippMartin E Hemler
Jan 19, 2010·Expert Reviews in Molecular Medicine·Christopher S Stipp
Jan 1, 2011·The AFCS-nature Molecule Pages·Trenis D Palmer, Andries Zijlstra
Feb 8, 2014·Nature Reviews. Cancer·Martin E Hemler
Sep 2, 2008·Nature Reviews. Drug Discovery·Martin E Hemler
Feb 3, 2005·Nature Reviews. Immunology·Shoshana Levy, Tsipi Shoham
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Martin E Hemler
Feb 20, 2010·Nature Reviews. Neuroscience·Yuko Fukata, Masaki Fukata
Nov 26, 2010·The Journal of Biological Chemistry·Christina M Van ItallieJames M Anderson
Oct 22, 2003·The Journal of Experimental Medicine·Louise A KoopmanJack L Strominger
Mar 31, 2006·Molecular Biology of the Cell·Nicole E WinterwoodChristopher S Stipp
May 30, 2008·Molecular Biology of the Cell·Chandan SharmaMartin E Hemler
Feb 13, 2009·Molecular Biology of the Cell·Marc A LafleurMartin E Hemler
Jul 10, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daosong XuMartin E Hemler
Apr 23, 2010·Photochemistry and Photobiology·Christopher R HayworthF Gonzalez-Lima
Jan 30, 2009·BMC Cell Biology·Susan FanayanJennifer A Byrne
Feb 5, 2008·BMC Genomics·Karine LambouMarc-Henri Lebrun
Jun 30, 2005·BMC Structural Biology·Oleg V KovalenkoMartin E Hemler
Jul 21, 2011·Journal of Cell Science·Hong-Xing WangMartin E Hemler
Feb 14, 2012·Journal of Cell Science·Xiuwei H YangMartin E Hemler
Oct 18, 2013·International Journal of Molecular Sciences·Bharat Bhusan PatnaikYeon Soo Han
Mar 14, 2014·Molecular Biology of the Cell·Christina M TerminiJennifer M Gillette
May 11, 2012·Cellular and Molecular Life Sciences : CMLS·Natalia Reglero-RealJaime Millán
Dec 17, 2008·Molecular Membrane Biology·Julie Charollais, F Gisou Van Der Goot
Nov 3, 2010·The Journal of Cell Biology·Andrew R FlanneryNorma W Andrews
Dec 22, 2004·The Journal of Cell Biology·Xiuwei YangMartin E Hemler
Dec 10, 2003·The Journal of Cell Biology·Christopher S StippMartin E Hemler
Dec 24, 2003·The Journal of Cell Biology·Nibedita ChattopadhyayJordan A Kreidberg
Aug 20, 2008·The Journal of Cell Biology·Cedric EspenelPierre-Emmanuel Milhiet
Oct 24, 2012·The Journal of Cell Biology·Emmanuel DornierEric Rubinstein
Oct 3, 2002·The Journal of Cell Biology·Alexander R KazarovMartin E Hemler
Oct 7, 2014·The New Phytologist·Piers A Hemsley
May 23, 2012·Plant Science : an International Journal of Experimental Plant Biology·Feng WangMieke Van Lijsebettens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.