Palmitoylcarnitine modulates interaction between protein kinase C betaII and its receptor RACK1

The FEBS Journal
Joanna Sobiesiak-Mirska, Katarzyna A Nałecz

Abstract

Palmitoylcarnitine, known to promote differentiation of neuroblastoma NB-2a cells as well as to inhibit protein kinase C (PKC) activity and to decrease phorbol ester binding, was shown previously to diminish the amount of complex formed between PKCdelta and its substrate GAP-43. In the present work we studied the effect of palmitoylcarnitine on the interaction between PKCbetaII and its receptor RACK1. Palmitoylcarnitine was found to decrease autophosphorylation of PKCbetaII on serine in a concentration-dependent manner and to decrease the amount of PKCbetaII/RACK1 complex. The effect of palmitoylcarnitine on cellular localization was found to be dependent on the presence of ATP; palmitoylcarnitine lowered the amount of PKCbetaII in cytosol and decreased the amount of PKCbetaII-RACK1 complex in membrane in the absence of ATP. Palmitoylcarnitine also reversed the effect of phorbol ester on the increase in the amount of PKCbetaII in membrane. Palmitoylcarnitine binds to PKCbetaII through hydrophobic interactions, although acylation of PKCbetaII by the palmitate moiety has been excluded. The presence of palmitoylcarnitine did not have any additive effect on the diminution of PKCbetaII-RACK1 complex formation in the presence of a RA...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Mochly-RosenJ Lopez
Aug 1, 1990·Trends in Pharmacological Sciences·R M Epand, D S Lester
Jan 1, 1990·Brain & Development·S Kimura, F Amemiya
Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Jan 1, 1990·Cellular Signalling·C House, B E Kemp
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Augusti-Tocco, G Sato
Oct 1, 1983·Physiological Reviews·J Bremer
Nov 1, 1982·Experimental Neurology·N BresolinC Angelini
Sep 10, 1995·Archives of Biochemistry and Biophysics·K A NałeczM J Nałecz
Oct 13, 1995·The Journal of Biological Chemistry·D RonD Mochly-Rosen
Aug 15, 1995·Biochemical and Biophysical Research Communications·A WawrzeńczykM J Nałecz
Jun 1, 1995·Neurochemistry International·A WawrzeńczykM J Nałecz
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Ron, D Mochly-Rosen
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·H KonishiY Nishizuka
Feb 12, 1998·Biochemical and Biophysical Research Communications·J E MroczkowskaK A Nalecz
Jun 13, 1998·The Journal of Biological Chemistry·N R Murray, A P Fields
Sep 28, 1998·Biochimica Et Biophysica Acta·A C Newton, J E Johnson
Oct 13, 1998·Current Biology : CB·M M ChouA Toker
Oct 30, 1998·Cellular Signalling·W S Liu, C A Heckman
Sep 10, 1999·The Journal of Biological Chemistry·D RonA Gordon
Jan 7, 2000·Biochemical and Biophysical Research Communications·J E MroczkowskaK A Naleçz
Feb 17, 2000·The EMBO Journal·D B ParekhP J Parker
Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Jaken, P J Parker
May 18, 2000·Biochemical Society Transactions·R R Ramsay
Jun 14, 2000·Molecular and Cellular Neurosciences·M VeitG Ahnert-Hilger
Jun 17, 2000·Biochimica Et Biophysica Acta·J Kerner, C Hoppel
Mar 30, 2001·European Journal of Biochemistry·A WawrzeńczykK A Nałecz
Mar 30, 2001·The Journal of Biological Chemistry·T MourtonS M Brady-Kalnay
May 15, 2001·Biochemical and Biophysical Research Communications·J A Koehler, M F Moran
Jun 2, 2001·The Journal of Biological Chemistry·E G Stebbins, D Mochly-Rosen
Jan 10, 2002·The Journal of Biological Chemistry·Lucia BanciDaria Mochly-Rosen

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