PMID: 8967426May 11, 1996Paper

Palytoxin modulates cytosolic pH in human osteoblast-like Saos-2 cells via an interaction with Na(+)-K(+)-ATPase

The American Journal of Physiology
J J Monroe, A H Tashjian

Abstract

Palytoxin (PTx) at nanomolar concentrations enhances the permeability of mammalian cell membranes to both Na+ and Ca2+. In basal human osteoblast-like Saos-2 cells, PTx (8 nM) caused a persistent decrease in cytosolic pH (pHi) of about 0.2 units, which required the presence of extracellular Ca2+ (Cae2+) and Na+ (Nae+). We acidified Saos-2 cells by incubation with nigericin to examine the action of PTx in cells with an activated Na+/H+ antiporter. Under these conditions, PTx increased the pHi without requiring Cae2+ or Nae+, and the alkalinization was unaffected by hexamethylene amiloride. We conclude that the PTx-induced rise in pHi did not involve the Na+/H+ antiporter. PTx increased the rate of 86Rb+ efflux. We propose that PTx induced alkalinization in nigericin-acidified cells by collapsing the K+ gradient. Exposure to ouabain had no effect on pHi, but it prevented the actions of PTx on PHi in both basal and nigericin-acidified cells. Ouabain-resistant mutant cells were less sensitive to PTx in extruding 86Rb+ than their ouabain-sensitive parents. We conclude that PTx interacts with the Na(+)-K(+)-adenosinetriphosphatase to regulate pHi in both basal and nigericin-acidified Saos-2 cells.

References

Nov 25, 1992·The Biochemical Journal·C S Graham, A H Tashjian
Feb 1, 1991·British Journal of Pharmacology·E Ecault, M P Sauviat
May 7, 1990·FEBS Letters·C FrelinJ P Breittmayer
Oct 15, 1985·European Journal of Biochemistry·H OzakiN Urakawa
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Jul 1, 1993·British Journal of Pharmacology·C van Renterghem, C Frelin

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Citations

Jun 16, 1997·FEBS Letters·X Wang, J D Horisberger
Nov 1, 2006·Journal of Neuroscience Research·Carmen Vale-GonzálezLuis M Botana
Nov 8, 2017·Archives of Toxicology·Jiri PatockaKamil Kuca
Oct 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·Gian Paolo Rossini, Albertino Bigiani
Jan 5, 2011·Toxicon : Official Journal of the International Society on Toxinology·Mirella BellocciSimone Prandi

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